Do aberrant crypt foci have predictive value for the occurrence of colorectal tumours? Potential of gene expression profiling in tumours

Autor: C.A.M Krul, Ruud Woutersen, M. J. van Erk, M.V.W Wijnands, Robert Doornbos
Rok vydání: 2004
Předmět:
Male
Pathology
cell-proliferation
Colorectal cancer
correlation analysis
Toxicology
medicine.disease_cause
human colon-cancer
CDK4
cyclin-dependent kinase 4
disease marker
Eating
azoxymethane-treated rats
AOM
azoxymethane
Intestinal mucosa
oncogene
curcumin
rat
RNA
Neoplasm
Intestinal Mucosa
ANOVA
analysis of variance
BIT
benzyl isothiocyanate
statistical significance
Benzyl isothiocyanate
Reverse Transcriptase Polymerase Chain Reaction
drug effect
article
General Medicine
rutoside
Gene Expression Regulation
Neoplastic
Real-time polymerase chain reaction
Tumor Markers
Biological
Biomarker (medicine)
dietary wheat bran
Colorectal Neoplasms
carcinogenesis
wheat bran
Aberrant crypt foci
chain fatty-acids
medicine.medical_specialty
DNA
Complementary
animal experiment
Triticum aestivum
antineoplastic activity
Biology
digestive system
intestine crypt
animal tissue
cDNA
complementary DNA
AIN
American Institute of Nutrition
Predictive Value of Tests
CA2
carbonic anhydrase II
medicine
gene expression profiling
Biomarkers
Tumor
Animals
Anticarcinogenic Agents
controlled study
protein expression
Toxicologie
Analytical research
VLAG
DNA Primers
nonhuman
Tissue Inhibitor of Metalloproteinase-1
Oncogene
AC
aberrant crypt
animal model
Gene Expression Profiling
induced intestinal carcinogenesis
Body Weight
ACF
aberrant crypt focus
scoring system
dehydrated citrus fiber
prediction
medicine.disease
Survival Analysis
Rats
Inbred F344
Diet
Rats
f344 rats
time series analysis
Cancer research
fischer-344 rats
benzyl isothiocyanate
tissue inhibitor
crypt cell
Carcinogenesis
dietary intake
Energy Metabolism
Food Science
colorectal tumor
Zdroj: Food and Chemical Toxicology, 10, 42, 1629-1639
Food and Chemical Toxicology 42 (2004) 10
Food and Chemical Toxicology, 42(10), 1629-1639
ISSN: 0278-6915
Popis: The effects of different dietary compounds on the formation of aberrant crypt foci (ACF) and colorectal tumours and on the expression of a selection of genes were studied in rats. Azoxymethane-treated male F344 rats were fed either a control diet or a diet containing 10% wheat bran (WB), 0.2% curcumin (CUR), 4% rutin (RUT) or 0.04% benzyl isothiocyanate (BIT) for 8 months. ACF were counted after 7, 15 and 26 weeks. Tumours were scored after 26 weeks and 8 months. We found that the WB and CUR diets inhibited the development of colorectal tumours. In contrast, the RUT and BIT diets rather enhanced (although not statistically significantly) colorectal carcinogenesis. In addition, the various compounds caused different effects on the development of ACF. In most cases the number or size of ACF was not predictive for the ultimate tumour yield. The expression of some tumour-related genes was significantly different in tumours from the control group as compared to tumours from the treated groups. It was concluded that WB and CUR, as opposed to RUT and BIT, protects against colorectal cancer and that ACF are unsuitable as biomarker for colorectal cancer. Effects of the different dietary compounds on metalloproteinase 1 (TIMP-1) expression correlated well with the effects of the dietary compounds on the ultimate tumour yield. © 2004 Elsevier Ltd. All rights reserved. Chemicals / CAS: benzyl isothiocyanate, 622-78-6; curcumin, 458-37-7; rutoside, 153-18-4, 22519-99-9; Anticarcinogenic Agents; DNA Primers; DNA, Complementary; RNA, Neoplasm; Tissue Inhibitor of Metalloproteinase-1; Tumor Markers, Biological
Databáze: OpenAIRE
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