Use of RNA-seq to determine variation in canine cytochrome P450 mRNA expression between blood, liver, lung, kidney and duodenum in healthy beagles
| Autor: | M. Visser, Gonzalo Rincon, Dawn A. Merritt, K. Weber |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Duodenum Biology Kidney digestive system Transcriptome 03 medical and health sciences 0302 clinical medicine Dogs Cytochrome P-450 Enzyme System Internal medicine Gene expression medicine Animals RNA Messenger Lung Pharmacology Messenger RNA General Veterinary Sequence Analysis RNA Kidney metabolism Cytochrome P450 respiratory system Molecular biology 030104 developmental biology medicine.anatomical_structure Endocrinology CYP2A13 Liver 030220 oncology & carcinogenesis biology.protein Female |
| Zdroj: | Journal of veterinary pharmacology and therapeutics. 40(6) |
| ISSN: | 1365-2885 |
| Popis: | RNA sequencing (RNA-seq) is a powerful tool for the evaluation and quantification of transcriptomes and expression patterns in animals, tissues, or pathological conditions. The purpose of this study was to determine the physiologic expression of cytochrome P450 (CYP) mRNA transcripts in whole blood, kidney, duodenum, liver, and lung in healthy, adult male (n = 4) and female (n = 4) beagles via RNA-seq. mRNA expression was above background (transcripts per million) for 45 canine CYPs, with liver, duodenum, and lung expressing a high number of xenobiotic metabolizing CYPs, while prominent endogenous metabolizing CYP expression was present in blood and kidney. The relative expression pattern of CYP2A13, 2B11, 2C21, 2D15, 2E1, 3A12, and 27A1 in liver, lung, and duodenum was verified through qPCR. This is the first global profiling of physiologic CYP mRNA expression in multiple canine tissues, providing a platform for further studies characterizing canine CYPs and changes in gene expression in disease states. |
| Databáze: | OpenAIRE |
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