The IFN-β 1b effect on Cu Zn superoxide dismutase (SOD1) in peripheral mononuclear blood cells of relapsing-remitting multiple sclerosis patients and in neuroblastoma SK-N-BE cells
Autor: | Anna Sasso, Vincenzo Bresciamorra, Bruna De Felice, A. Belfiore, Mariarosaria Santillo, Antonio Carotenuto, Nicola Salvatore Orefice, Paolo Mondola, Simona Damiano, G. Vacca, Giuseppe Terrazzano, Giuseppe Orefice |
---|---|
Přispěvatelé: | Damiano, Simona, Sasso, Anna, DE FELICE, Bruna, Terrazzano, Giuseppe, Bresciamorra, Vincenzo, Carotenuto, Antonio, Orefice, Nicola S., Orefice, Giuseppe, Vacca, Giovanni, Belfiore, Annamaria, Santillo, Mariarosaria, Mondola, Paolo, De Felice, Bruna, BRESCIA MORRA, Vincenzo |
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Adult
Male SOD1 Blotting Western Inflammation Enzyme-Linked Immunosorbent Assay Superoxide dismutase Peripheral blood mononuclear cell Neuroblastoma Multiple Sclerosis Relapsing-Remitting Superoxide Dismutase-1 Interferon Cell Line Tumor Medicine Humans RNA Messenger Neuroscience (all) biology business.industry General Neuroscience Multiple sclerosis Interferon beta-1b medicine.disease Case-Control Studies Immunology biology.protein Leukocytes Mononuclear Female medicine.symptom Case-Control Studie business Relapsing-remitting multiple sclerosis (RR-MS) Human medicine.drug |
Popis: | Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease leading to axonal injury. Even if the etiology of MS is still unknown the disease begins with inflammation involving autoreactive T lymphocytes activation in genetically susceptible subjects. Interferon beta-1b (IFN β 1b) is one of the most used drug in the MS therapy. The results obtained in this study show that the concentration of SOD1 in CSF of relapsing-remitting MS (RR-MS) patients, evaluated by enzyme-linked immunosorbent assay (ELISA), is decreased compared to pathological controls. Moreover, the Western blotting analysis demonstrated that SOD1 in human peripheral blood mononuclear cells (PBMC) in healthy controls was significantly higher compared to MS subjects before starting DMT therapy. In addition IFN β 1b therapy causes an increase of intracellular SOD1 protein as well as mRNA levels in PBMC. Moreover, the treatment of neuroblastoma SK-N-BE cells with IFN β 1b increased SOD1 protein and mRNA levels; these data also suggest that neuroprotective effect of this physiological molecule is, at least in part, carried out through its effect on SOD1. This study demonstrate that DMT therapy is able to increase SOD1 expression in PBMC of RR-MS patients. Therefore, the effectiveness of DMT therapy can be ascribed, at least in part, to an increased levels of this antioxidant enzyme as further confirmed by in vitro studies in SK-N-BE cells. |
Databáze: | OpenAIRE |
Externí odkaz: |