Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat
| Autor: | C. Valon, H. Recipon, Yves Henry, E. Delcher-Besin, Bernard Lejeune, J. De Buyser, Marie-France Jubier, Caroline Hartmann, A. Rode |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Mitochondrial DNA
Molecular Sequence Data Restriction Mapping DNA Mitochondrial DNA sequencing Restriction fragment chemistry.chemical_compound Sequence Homology Nucleic Acid Genetics Regeneration Cloning Molecular Repeated sequence Triticum Genomic organization DNA Primers Repetitive Sequences Nucleic Acid Recombination Genetic biology Base Sequence Genetic Variation General Medicine Gene rearrangement chemistry biology.protein Restriction fragment length polymorphism DNA |
| Zdroj: | Current genetics. 25(5) |
| ISSN: | 0172-8083 |
| Popis: | The mitochondrial genome of the selfed progeny of a plant regenerated from long-term somatic tissue culture displays specific structural rearrangements characterized by the appearance of novel restriction fragments. A mitochondrial DNA library was constructed from this selfed progeny in the SalI site of cosmid pHC79 and the novel fragments were subsequently studied. They were shown to arise from reciprocal recombination events involving DNA sequences present in the parental plant. The regions of recombination were sequenced and the nucleotide sequences were aligned with those of the presumptive parental fragments. We characterized an imperfect short repeated DNA sequence, 242 bp long, within which a 7-bb DNA repeat could act as a region of recombination. The use of PCR technology allowed us to show that these fragments were present in both parental plants and tissue cultures as low-abundance sequence arrangements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink