Virus-like particle nanoreactors: programmed en capsulation of the thermostable CelB glycosidase inside the P22 capsid
| Autor: | Trevor Douglas, John van der Oost, Peter E. Prevelige, Dustin P. Patterson, Benjamin Schwarz, Kheireddine El-Boubbou |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
archaeon pyrococcus-furiosus
Immobilized enzyme viruses Nanotechnology Nanoreactor complex mixtures Microbiology Nanomaterials Bacteriophage Virus-like particle protein cages Microbiologie silica immobilization scaffolding protein Glycoside hydrolase bacteriophage p22 VLAG enzyme immobilization biology Chemistry virus diseases General Chemistry Condensed Matter Physics biology.organism_classification Capsid Colloidal gold gold nanoparticles Biophysics escherichia-coli functional domains beta-glucosidase celb |
| Zdroj: | Soft Matter, 8(39), 10158-10166 Soft Matter 8 (2012) 39 |
| ISSN: | 1744-6848 |
| Popis: | Self-assembling biological systems hold great potential for the synthetic construction of new active soft nanomaterials. Here we demonstrate the hierarchical bottom-up assembly of bacteriophage P22 virus-like particles (VLPs) that encapsulate the thermostable CelB glycosidase creating catalytically active nanoreactors. The in vivo assembly and encapsulation produces P22 VLPs with a high packaging density of the tetrameric CelB, but without loss of enzyme activity or the ability of the P22 VLP to undergo unique morphological transitions that modify the VLPs internal volume and shell porosity. The P22 VLPs encapsulating CelB are also shown to retain a high percentage of the enzyme activity upon being embedded and immobilized in a polymeric matrix. |
| Databáze: | OpenAIRE |
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