A Peroxisome-Inspired Chemiluminescent Silica Nanodevice for the Intracellular Detection of Biomarkers and Its Application to Insulin-Sensitizer Screening
| Autor: | Yue Zhang, Haimei Yang, Zhining Xia, Weili Wei, Min Wang, Xu Jie |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Luminescence
medicine.medical_treatment 02 engineering and technology 010402 general chemistry 01 natural sciences Catalysis law.invention chemistry.chemical_compound Mice Microscopy Electron Transmission law medicine Peroxisomes Animals Humans Insulin Lactic Acid Nanodevice Chemiluminescence Ethanol Chemistry Drug discovery General Medicine General Chemistry Hep G2 Cells Peroxisome 021001 nanoscience & nanotechnology Silicon Dioxide 0104 chemical sciences Lactic acid Uric Acid Glucose Biochemistry Luminescent Measurements Uric acid 0210 nano-technology Intracellular Biomarkers |
| Zdroj: | Angewandte Chemie (International ed. in English). 56(46) |
| ISSN: | 1521-3773 |
| Popis: | Profiling of oxidase-catalyzed biomarkers is an essential procedure for the diagnosis and precise treatment of the metabolic diseases. Inspired by the metabolism of H2O2 in peroxisomes, a novel chemiluminescent silica nanodevice (CSN) was designed for sensitive and selective sensing of oxidase-catalyzed biomarkers. Oxidases catalyzed the oxidation of biomarkers followed by the production of H2O2, and then the generated H2O2 was employed as a messenger to trigger the chemiluminescence of the CSN. Utilizing this nanodevice, we not only accurately quantified intracellular glucose but also developed its further application for high throughput insulin sensitizer screening. Furthermore, sensitive and multiparametric analysis of oxidase-catalyzed biomarkers like lactic acid, uric acid and ethanol was also demonstrated. Thus, this peroxisome-inspired CSN holds great promise in the general diagnosis of metabolic diseases and drug discovery. |
| Databáze: | OpenAIRE |
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