Correlation between phenotypes of restriction / modification systems and efficiency of transformation in Campylobacter jejuni
| Autor: | Masaaki Minami, Hidemi Goto, Takafumi Ando, Michio Ohta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Genetics
education.field_of_study Campylobacter jejuni Transformation efficacy Restriction modification Horizontal gene transfer Natural competent biology Population biology.organism_classification Transformation (genetics) Restriction enzyme Agarose gel electrophoresis Pulsed-field gel electrophoresis education Gene Transformation efficiency |
| DOI: | 10.5281/zenodo.5599692 |
| Popis: | We evaluated the effect of restriction and modification genes expressed inCampylobacter jejuni, a naturally competent organism, on transformation efficiency. Fourteen strains ofC. jejuniwere tested for sensitivity to 29 restriction enzymes. Strains that showed the same susceptibility pattern by either agarose gel electrophoresis or PFGE were grouped together. Natural transformation between strains within and between groups was assessed using two chromosomal markers, one an artificially introduced gene and the other a selected point mutation. However, no correlation was found between transformation efficiency and sensitivity to the restriction enzymes tested. The activity of the restriction and modification system alone could not explain the failure of transformation to occur or the generation of stable clones in a population ofC. jejuniwith other abilities. It is likely that other factors influence horizontal gene flow in this organism. We suggested that the rule that the restriction and modification system affect horizontal gene flow, based on studies of transfection withE. coli, may not apply to the exchange of chromosomal DNA inC. jejuni. |
| Databáze: | OpenAIRE |
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