Transplantation of Bioreactor-Produced Neural Stem Cells into the Rodent Brain
Autor: | Murray Hong, Marcus McLeod, R. Ulalia, Arindom Sen, Leo A. Behie, Ivar Mendez, D. Sadi |
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Rok vydání: | 2006 |
Předmět: |
0301 basic medicine
Time Factors Rodent Cell Survival Cell Biomedical Engineering lcsh:Medicine Cell Line Mice 03 medical and health sciences Bioreactors 0302 clinical medicine Neurosphere biology.animal Glial Fibrillary Acidic Protein medicine Bioreactor Animals Rats Wistar reproductive and urinary physiology Neurons Transplantation Microscopy Confocal biology Glial fibrillary acidic protein Stem Cells lcsh:R Brain Cell Differentiation Cell Biology Immunohistochemistry Neural stem cell Rats nervous system diseases Neuroepithelial cell Ki-67 Antigen 030104 developmental biology medicine.anatomical_structure nervous system biology.protein Female biological phenomena cell phenomena and immunity Neuroscience 030217 neurology & neurosurgery Stem Cell Transplantation |
Zdroj: | Cell Transplantation, Vol 15 (2006) |
ISSN: | 1555-3892 0963-6897 |
Popis: | The development of new cell replacement strategies using neural stem cells (NSC) may provide an alternative and unlimited cell source for clinical neural transplantation in neurodegenerative diseases such as Parkinson's and Huntington's disease. The clinical application of neural transplantation using NSC will therefore depend upon the availability of clinical grade NSC that are generated in unlimited quantities in a standardized manner. In order to investigate the utility of NSC in clinical neural transplantation, undifferentiated murine NSC were first expanded for an extended period of time in suspension bioreactors containing a serum-free medium. Following expansion in suspension bioreactors, NSC were still able to differentiate in vitro into both astrocytes and neurons after exposure to brain-derived neurotrophic factor (BDNF), suggesting that bioreactor expansion does not alter cell lineage potentiality. Undifferentiated bioreactor-expanded NSC were then transplanted into the rodent striatum. Immunohistochemical examination revealed undifferentiated bioreactor-expanded NSC survived transplantation for up to 8 weeks and expressed the astrocytic immunohistochemical marker glial fibrillary acidic protein (GFAP), suggesting that the host striatal environment influences NSC cell fate upon transplantation. Moreover, no tumor formation was observed within the graft site, indicating that NSC expanded in suspension bioreactors for an extended period of time are a safe source of tissue for transplantation. Future studies should focus on predifferentiating NSC towards specific neuronal phenotypes prior to transplantation in order to restore behavioral function in rodent models of neurodegenerative disease. |
Databáze: | OpenAIRE |
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