Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

Autor: Selcuk Kilic, Aylin Ersoy, Sumeyra Savas, Zeynep Altintas, Belkıs Levent, Yakup Gulmez
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Serotype
Salmonella
02 engineering and technology
medicine.disease_cause
infectious diseases
01 natural sciences
lcsh:Technology
Typhoid fever
Microbiology
medicine
General Materials Science
lcsh:Microscopy
lcsh:QC120-168.85
Detection limit
microfluidic-based electrochemical sensor
nanoparticle enhanced bio-detection
medicine.diagnostic_test
biology
lcsh:QH201-278.5
Chemistry
lcsh:T
010401 analytical chemistry
021001 nanoscience & nanotechnology
medicine.disease
biology.organism_classification
pathogen detection
0104 chemical sciences
540 Chemie und zugeordnete Wissenschaften
lcsh:TA1-2040
Immunoassay
antibody biosensor
ddc:540
Salmonella spp
biology.protein
lcsh:Descriptive and experimental mechanics
lcsh:Electrical engineering. Electronics. Nuclear engineering
Antibody
0210 nano-technology
lcsh:Engineering (General). Civil engineering (General)
Biosensor
lcsh:TK1-9971
Bacteria
DNA biosensor
Zdroj: Materials, Vol 11, Iss 9, p 1541 (2018)
Materials
Volume 11
Issue 9
ISSN: 1996-1944
Popis: Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL&minus
1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002&ndash
200 µ
M using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection.
Databáze: OpenAIRE
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