In vitro and in vivo studies of ultrafine-grain Ti as dental implant material processed by ECAP
| Autor: | Zhirui Li, Xiaoou Diao, Haitao Xin, Zhang Qiang, Jia Xiaorui, Baili An, Yulu Wu, Kai Li, Yazhou Guo |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Biocompatibility Surface Properties medicine.medical_treatment chemistry.chemical_element Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Osseointegration Biomaterials In vivo Materials Testing medicine Cell Adhesion Animals Dental implant Cell Proliferation Dental Implants Titanium Metallurgy Adhesion 021001 nanoscience & nanotechnology Microstructure 0104 chemical sciences chemistry Mechanics of Materials Implant Rabbits 0210 nano-technology Biomedical engineering |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 67 |
| ISSN: | 1873-0191 |
| Popis: | The aim of this study was to investigate the surface characterization of ultrafine-grain pure titanium (UFG-Ti) after sandblasting and acid-etching (SLA) and to evaluate its biocompatibility as dental implant material in vitro and in vivo. UFG-Ti was produced by equal channel angular pressing (ECAP) using commercially pure titanium (CP-Ti). Microstructure and yield strength were investigated. The morphology, wettability and roughness of the specimens were analyzed after they were modified by SLA. MC3T3-E1 osteoblasts were seeded onto the specimens to evaluate its biocompatibility in vitro. For the in vivo study, UFG-Ti implants after SLA were embedded into the femurs of New Zealand rabbits. Osseointegration was investigated though micro-CT analysis, histological assessment and pull-out test. The control group was CP-Ti. UFG-Ti with enhanced mechanical properties was produced by four passes of ECAP in BC route at room temperature. After SLA modification, the hierarchical porous structure on its surface exhibited excellent wettability. The adhesion, proliferation and viability of cells cultured on the UFG-Ti were superior to that of CP-Ti. In the in vivo study, favorable osseointegration occurred between the implant and bone in CP and UFG-Ti groups. The combination intensity of UF- Ti with bone was higher according to the pull-out test. This study supports the claim that UFG-Ti has grain refinement with outstanding mechanical properties and, with its excellent biocompatibility, has potential for use as dental implant material. |
| Databáze: | OpenAIRE |
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