Hepatic stimulator substance activity in the liver of thioacetamide-intoxicated rats
Autor: | Alexandra Margeli, Stamatios Theocharis, Spyridon D. Skaltsas, Michael G. Mykoniatis, Chara Spiliopoulou |
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Rok vydání: | 2000 |
Předmět: |
Male
medicine.medical_specialty Mitotic index medicine.medical_treatment Biology Thioacetamide Thymidine Kinase chemistry.chemical_compound Internal medicine medicine Mitotic Index Animals Hepatectomy Aspartate Aminotransferases Rats Wistar Growth Substances Liver injury integumentary system Hepatology Growth factor Alanine Transaminase DNA medicine.disease Liver regeneration Liver Regeneration Rats medicine.anatomical_structure Endocrinology chemistry Alanine transaminase Liver Hepatocyte biology.protein Intercellular Signaling Peptides and Proteins Chemical and Drug Induced Liver Injury Peptides Injections Intraperitoneal Thymidine |
Zdroj: | Liver. 19(6) |
ISSN: | 0106-9543 |
Popis: | Aims/Background: Hepatic stimulator substance (HSS) is a known hepatic growth factor which appears to be organ-specific but species non-specific. We have recently shown that the administration of HSS enhanced hepatocyte proliferation occurring due to thioacetamide (TAA)-induced liver injury in rats (Theocharis SE, et al., Scand J Gastroenterol 1998; 33: 656–63). In the present study, we examined the activity of the endogenously produced HSS in the liver of TAA administered rats during injury and regeneration. Methods: TAA at a dose of 300 mg/kg of body weight was injected intraperitoneally in male Wistar rats. The animals were sacrificed at 0, 12, 24, 36, 48, 60 and 72 h after TAA administration. The rate of tritiated thymidine incorporation into hepatic DNA, the enzymatic activity of liver thymidine kinase and the assessment of mitotic index in hepatocytes were used to estimate liver regeneration. HSS extract was obtained from the livers of TAA-treated rats, sacrificed at the above mentioned time points. This HSS extract was injected in 34% partially hepatectomized rats, to assess its activity. The ability of the injected HSS extract to increase hepatocellular proliferation over that normally occurring 24 h following 34% partial hepatectomy was used to express the activity of HSS by determining the above mentioned indices of liver regeneration. Results: The administration of TAA caused severe hepatic injury recognized histopathologically as well as by the increased activities of serum hepatic enzymes aspartate and alanine aminotrasferases. The hepatic injury, which peaked at 24 and 36 h post-TAA treatment (p |
Databáze: | OpenAIRE |
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