Cone Degeneration Following Rod Ablation in a Reversible Model of Retinal Degeneration
| Autor: | Gustav A. Engbretson, Michael E. Zuber, Ilija Aleksic, Adam Coughlin, Georgette A. Jones, Rene Y. Choi, Eduardo Solessio |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Retinal degeneration Calbindins Genotype genetic structures Vision Disorders Xenopus Apoptosis Cell Count Retinal Cone Photoreceptor Cells Gene Expression Regulation Enzymologic Animals Genetically Modified Xenopus laevis S100 Calcium Binding Protein G Retinal Rod Photoreceptor Cells Metronidazole Retinitis pigmentosa In Situ Nick-End Labeling medicine Animals Regeneration Fluorescent Antibody Technique Indirect In Situ Hybridization Fluorescence Retinal regeneration Genetics Retina biology Escherichia coli Proteins Articles Nitroreductases medicine.disease biology.organism_classification Cell biology Disease Models Animal medicine.anatomical_structure Microscopy Fluorescence Rhodopsin biology.protein Female sense organs Neuroglia Retinitis Pigmentosa |
| Zdroj: | Investigative Opthalmology & Visual Science. 52:364 |
| ISSN: | 1552-5783 |
| Popis: | Amphibian retinas regenerate after injury, making them ideal for studying the mechanisms of retinal regeneration, but this leaves their value as models of retinal degeneration in question. The authors asked whether the initial cellular changes after rod loss in the regenerative model Xenopus laevis mimic those observed in nonregenerative models. They also asked whether rod loss was reversible.The authors generated transgenic X. laevis expressing the Escherichia coli enzyme nitroreductase (NTR) under the control of the rod-specific rhodopsin (XOP) promoter. NTR converts the antibiotic metronidazole (Mtz) into an interstrand DNA cross-linker. A visually mediated behavioral assay and immunohistochemistry were used to determine the effects of Mtz on the vision and retinas of XOPNTR F1 tadpoles.NTR expression was detected only in the rods of XOPNTR tadpoles. Mtz treatment resulted in rapid vision loss and near complete ablation of rod photoreceptors by day 12. Müller glial cell hypertrophy and progressive cone degeneration followed rod cell ablation. When animals were allowed to recover, new rods were born and formed outer segments.The initial secondary cellular changes detected in the rodless tadpole retina mimic those observed in other models of retinal degeneration. The rapid and synchronous rod loss in XOPNTR animals suggested this model may prove useful in the study of retinal degeneration. Moreover, the regenerative capacity of the Xenopus retina makes these animals a valuable tool for identifying the cellular and molecular mechanisms at work in lower vertebrates with the remarkable capacity of retinal regeneration. |
| Databáze: | OpenAIRE |
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