Diffusion Tensor Imaging of Visual Pathway Abnormalities in Five Glaucoma Animal Models
| Autor: | Xiaoling Yang, Gadi Wollstein, John H. Fingert, Leon C. Ho, Yiqin Du, Aaron S. Field, Muneeb A. Faiq, Gillian J. McLellan, Kevin C. Chan, Joel S. Schuman, Carlos Parra, Max K. Colbert, Hongmin Yun, Samuel A. Hurley, Ian P. Conner, Yolandi van der Merwe |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Intraocular pressure Optic tract genetic structures Glaucoma Optic neuropathy Rats Sprague-Dawley Mice Nerve Fibers Multidisciplinary Ophthalmic Imaging Ophthalmology Fractional anisotropy Genetic model medicine magnetic resonance imaging Animals Visual Pathways visual pathway Gray Matter Intraocular Pressure business.industry Optic Nerve medicine.disease diffusion tensor imaging eye diseases optic neuropathy Rats Disease Models Animal medicine.anatomical_structure glaucoma Mice Inbred DBA Optic nerve Cats Anisotropy Trabecular meshwork sense organs business Glaucoma Open-Angle |
| Zdroj: | Investigative Ophthalmology & Visual Science |
| ISSN: | 1552-5783 0146-0404 |
| Popis: | Purpose To characterize the visual pathway integrity of five glaucoma animal models using diffusion tensor imaging (DTI). Methods Two experimentally induced and three genetically determined models of glaucoma were evaluated. For inducible models, chronic IOP elevation was achieved via intracameral injection of microbeads or laser photocoagulation of the trabecular meshwork in adult rodent eyes. For genetic models, the DBA/2J mouse model of pigmentary glaucoma, the LTBP2 mutant feline model of congenital glaucoma, and the transgenic TBK1 mouse model of normotensive glaucoma were compared with their respective genetically matched healthy controls. DTI parameters, including fractional anisotropy, axial diffusivity, and radial diffusivity, were evaluated along the optic nerve and optic tract. Results Significantly elevated IOP relative to controls was observed in each animal model except for the transgenic TBK1 mice. Significantly lower fractional anisotropy and higher radial diffusivity were observed along the visual pathways of the microbead- and laser-induced rodent models, the DBA/2J mice, and the LTBP2-mutant cats compared with their respective healthy controls. The DBA/2J mice also exhibited lower axial diffusivity, which was not observed in the other models examined. No apparent DTI change was observed in the transgenic TBK1 mice compared with controls. Conclusions Chronic IOP elevation was accompanied by decreased fractional anisotropy and increased radial diffusivity along the optic nerve or optic tract, suggestive of disrupted microstructural integrity in both inducible and genetic glaucoma animal models. The effects on axial diffusivity differed between models, indicating that this DTI metric may represent different aspects of pathological changes over time and with severity. |
| Databáze: | OpenAIRE |
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