Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats
| Autor: | Hao Wang, Bin He, Jianda Zhou, Jia Chen, Ping Li, Shaohua Wang, Liang Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cellular differentiation Biophysics Down-Regulation Bone Marrow Cells wound healing Fibroblast growth factor Biochemistry Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine stomatognathic system Epidermal growth factor Basic Helix-Loop-Helix Transcription Factors Animals rat Viability assay Tcf3 Wnt Signaling Pathway Molecular Biology beta Catenin Research Articles Cell Proliferation Skin Skin repair integumentary system Chemistry Cell growth Mesenchymal stem cell bone marrow mesenchymal stem cells Cell Differentiation Epithelial Cells Mesenchymal Stem Cells Cell Biology Rats Cell biology 030104 developmental biology 030220 oncology & carcinogenesis embryonic structures Wound healing Signal Transduction Research Article |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | The aim of the present study was to explore the wound healing effect of Tcf3 in rat bone marrow mesenchymal stem cells (BMSCs) and their effects on wound healing. Epidermal growth factor (EGF) and fibroblast growth factors (FGFs) were used to induce BMSCs differentiation into epithelial-like cells. Western Blotting analysis and RT-qPCR were performed to assess the expression levels of Tcf3 and the markers of epithelial-like cells, such as Cytokeratin-18 (CK-18), CK-19 and P63. Cell counting kit-8 (CCK-8) and clone formation assay were carried out to detect cell viability. Immunohistochemistry and HE staining were used to assess the level of Tcf3 protein and skin repair degree, respectively. Rat wound healing model was built to evaluate the effects of BMSCs with altered expression of Tcf3 on wound healing. Results showed that EGF and FGFs stimulation increased the expression of CK-18, CK-19 and P63, improved BMSCs viability, but decreased the expression of Tcf3. Knockdown of Tcf3 in BMSCs increased CK-18, CK-19 and P63 expression and improved cell proliferation, as well as accelerated wound healing process. Moreover, inhibition of Wnt/β-catenin signaling weakened the effect of Tcf3 down-regulation on BMSCs proliferation enhancement. And inhibition of Notch1 signaling impeded the epithelial-like cell differentiation of BMSCs induced by Tcf3 down-regulation. Our study reveals that knockdown of Tcf3 enhances the wound healing process of BMSCs in rat, which provides new approach for accelerating skin regeneration. |
| Databáze: | OpenAIRE |
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