Antigen expression and polymerase chain reaction amplification of mantle cell lymphomas
| Autor: | RJ Molot, TC Meeker, CT Wittwer, SL Perkins, GH Segal, AS Masih, RC Braylan, CR Kjeldsberg |
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| Rok vydání: | 1994 |
| Předmět: |
Aged
80 and over Chromosomes Human Pair 14 Male Base Sequence Chromosomes Human Pair 11 Immunology Molecular Sequence Data Cell Biology Hematology DNA Neoplasm Middle Aged Flow Cytometry Biochemistry Polymerase Chain Reaction Translocation Genetic immune system diseases hemic and lymphatic diseases Antigens Surface Humans Female neoplasms Lymphoma Follicular Aged |
| Zdroj: | Blood. 83(6) |
| ISSN: | 0006-4971 |
| Popis: | Flow immunophenotyping, DNA content analysis, and polymerase chain reaction (PCR) amplification for t(11;14) and t(14;18) were performed on 11 cases of typical mantle cell lymphoma (MCL), 5 cases of apparent MCL with proliferation centers (MCL-PC), and 5 cases of small lymphocytic lymphoma (SLL). Immunophenotyping showed IgM (P < .001), Ig light (P < .001), and CD20 (P < .001) expression to be more intense in MCL than in SLL. In MCL-PC, the mean intensity of IgM, Ig light chain, and CD20 expression was intermediate to the intensities observed in MCL and SLL. Furthermore, in contrast to SLL, all MCL and 4 of 5 MCL-PC cases exhibited stronger CD20 than CD19 expression. CD10 expression was not observed in any case and CD5 expression was present in all SLL and MCL-PC cases and in 9 of 11 MCL cases. DNA content analysis showed an S- phase fraction of less than 3% in all cases studied and, except for 1 MCL case, all lymphomas were DNA diploid. The t(11;14) breakpoint junctions involving the bcl-1 major translocation cluster were amplified by PCR in 4 of 11 (36%) MCL cases and in none of the MCL-PC or SLL cases. The t(14;18) involving the bcl-2 major breakpoint region was not identified by PCR in any case. We conclude that the level of expression of surface antigens and the rapid detection of t(11;14) by PCR are potentially useful for distinguishing MCL and SLL in the clinical setting. Further investigations as to the biologic relationship between MCL, MCL-PC, and SLL, and the utility of t(11;14) PCR in these lymphomas are warranted. |
| Databáze: | OpenAIRE |
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