Antiviral and immunomodulating activities of chemically synthesized lipid A-subunit analogues GLA-27 and GLA-60
| Autor: | Chiaki Nishimura, Mitsunobu Nakatsuka, Makoto Kiso, Akira Hasegawa, J. Yuzuru Homma, S. Ikeda |
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| Rok vydání: | 1988 |
| Předmět: |
Male
Interferon Inducers Lipopolysaccharide Vaccinia virus In Vitro Techniques Antiviral Agents Virus Lipid A chemistry.chemical_compound Mice Adjuvants Immunologic Interferon Glucosamine Virology medicine Animals cardiovascular diseases Orthopoxvirus Pharmacology biology nutritional and metabolic diseases Biological activity Macrophage Activation biology.organism_classification Molecular biology Killer Cells Natural chemistry Biochemistry lipids (amino acids peptides and proteins) Female Rabbits Cell activation Acetylmuramyl-Alanyl-Isoglutamine hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Antiviral research. 9(1-2) |
| ISSN: | 0166-3542 |
| Popis: | Biological and antiviral activities of chemically synthesized lipid A-subunit analogues, GLA-27 and GLA-60, were investigated with respect to defense mechanisms such as macrophage and natural killer (NK) cell activation and interferon (IFN)-indluding activity. GLA-27, a 4-O- phosphono - D - glucosamine derivative carrying 3-O-tetradecanoyl (C14) and 2-N-3-tetradecanoyloxytetradecanoyl (C14-O-(C14)) group, and GLA-60, a similar analogue carrying 3-O-linked C14-O-(C14) and 3-N-linked 3-hydroxytetradecanoyl (C14-OH) groups, strongly inhibited the formation of pox tail lesions and the growth of vaccinia virus at the tail lesion sites in infected mice. The antiviral activity of GLA-60 was about 1000-fold higher than that of muramyldipeptide (MDP), a representative immunomodulator. GLA-27 and GLA-60 had stronger immunomodulating activity than MDP in macrophage activation, NK cell activation and IFN-inducing activity, although it was weaker than natural lipid A. Toxic manifestations such as pyrogenicity, local Shwartzman reaction and lethality were far less pronounced for GLA-27 and GLA-60 than for natural lipid A. |
| Databáze: | OpenAIRE |
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