Gene silencing results in instability of antibody production in transgenic plants
| Autor: | Anna Depicker, I Strobbe, H. Van Houdt, M. De Neve, M. Van Montagu, Anni Jacobs, S. De Buck, C De Wilde |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial Chalcone synthase Transcription Genetic biology Transgene Arabidopsis Methylation DNA Methylation Genes Plant Plants Genetically Modified biology.organism_classification Molecular biology Plant Leaves Immunoglobulin Fab Fragments Transformation Genetic Gene Expression Regulation Plant Antibody Formation DNA methylation Genetics biology.protein Gene silencing Epigenetics Antibody Molecular Biology |
| Zdroj: | Molecular and General Genetics MGG. 260:582-592 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/s004380050932 |
| Popis: | The stability of antibody and Fab expression was assessed in five different homozygous transgenic Arabidopsis lines. Each of these lines showed silencing of the transgenes that encode the antibody polypeptides, leading to instability of antibody production. However, each line had a different and specific instability profile. The characteristic variation in the level of antibody accumulation in each line as a function of developmental stage indicated that the T-DNA integration pattern played a role in triggering silencing, and also that the history and the integration position of simple transgene loci can influence the susceptibility to epigenetic silencing. In different lines with low antibody accumulation levels, methylation was found either in the promoter alone, in both the promoter and the transcribed region, in the transcribed region only, or in the transcribed region and downstream sequences. In conclusion, our data suggest that epigenetic effects result in different transgene expression profiles in each of the five Arabidopsis lines analyzed. |
| Databáze: | OpenAIRE |
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