Expression of p97/VCP and ubiquitin during postnatal development of the degenerating rat retina
| Autor: | Helin Deniz Demir, Sevil Cayli, Hüseyin Ortak, Seda Ocakli, Erkan Söğüt, Ufuk Taş |
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| Rok vydání: | 2011 |
| Předmět: |
Retinal degeneration
Histology genetic structures Physiology Gene Expression Cell Cycle Proteins Biology Retina Ubiquitin Valosin Containing Protein medicine Animals Rats Wistar Outer nuclear layer Ganglion cell layer Adenosine Triphosphatases Retinal pigment epithelium Retinal Degeneration Gene Expression Regulation Developmental Colocalization Cell Biology General Medicine medicine.disease Molecular biology eye diseases Rats medicine.anatomical_structure Inner nuclear layer biology.protein sense organs Protein Binding |
| Zdroj: | Journal of Molecular Histology. 43:17-25 |
| ISSN: | 1567-2387 1567-2379 |
| DOI: | 10.1007/s10735-011-9374-y |
| Popis: | In this study, we aimed to investigate the distribution pattern of ubiquitin and p97/VCP in the rat retina during postnatal development. Eyeballs from 1-, 4-, 10-, 36- and 72-week-old rats were examined by immunohistochemistry, and protein colocalization was determined by immunofluorescence microscopy. In the 1-week-old rat retina, p97/VCP was strongly expressed in the neuroblast layer, however no ubiquitin immunoreactivity was observed. p97/VCP immunoreactivity was present in the ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS) of the photoreceptor layer, and retinal pigment epithelium in the 4- and 10-week-old rat retinas. p97/VCP immunoreactivity increased significantly in the 10-week-old rat retinas. Ubiquitin was barely seen in the 4-week-old rat retinas, and ubiquitin expression was weak in the GCL and the IPL of the 10-week-old rat retinas. In the 36- and 72-week-old rats, the presence of ubiquitin was remarkable in the IS, INL, IPL and GCL, however, p97/VCP immunoreactivity was significantly decreased. Colocalization of ubiquitin and p97/VCP was also observed in the INL, IS, GCL and ONL of 36- and 72-week-old rat retinas. Our results indicate that p97/VCP immunoreactivity in the retina significantly decreases after rats reach 10 weeks of age, whereas ubiquitin immunoreactivity increases with aging. These results suggest that an altered expression pattern of p97/VCP and ubiquitin in the developing rat retina may associate with age-related retinal degeneration. |
| Databáze: | OpenAIRE |
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