Overfeeding Can Cause NEC-Like Enterocolitis in Premature Rat Pups
| Autor: | Yoko Yamakawa, Tohru Fujii, Hirohisa Izumi, Yuichiro Yamashiro, Yoshikazu Ohtsuka, Toshiaki Shimizu, Kyo Okada |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Inflammation Infant Premature Diseases Biology Gastroenterology Rats Sprague-Dawley Overnutrition NF-KappaB Inhibitor alpha Enterocolitis Necrotizing Pregnancy Internal medicine medicine Animals Humans Intestinal Mucosa Enterocolitis Neonatal necrotizing enterocolitis Mortality rate Infant Newborn medicine.disease digestive system diseases Animals Suckling Rats PPAR gamma Sprague dawley Disease Models Animal Animals Newborn Pediatrics Perinatology and Child Health Immunology Female I-kappa B Proteins medicine.symptom Developmental Biology |
| Zdroj: | Neonatology. 97:218-224 |
| ISSN: | 1661-7819 1661-7800 |
| DOI: | 10.1159/000253150 |
| Popis: | Background: Neonatal necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in premature infants. The mortality rate associated with NEC is quite high and in most reports ranges from 20 to 30%. Despite extensive studies, the pathogenesis of NEC remains poorly understood. Objectives: To investigate the mechanisms of NEC in terms of inflammatory signaling in the intestine. Methods: A new enterocolitis model was established and examined the expression of inflammatory and anti-inflammatory signals in the intestines of rat pups. The premature rat pups, delivered by abdominal incision on day 20 of gestation (day 21 is considered as full term), were divided into three groups, and they were given a single administration of 0.05, 0.1, and 0.15 ml of formula milk via an orogastric catheter. After 24 h, the development of enterocolitis was evaluated by the presence of hemorrhagic enterocolitis, and the expression of signaling molecules, inhibitor of nuclear factor-ĸB (IĸB)-α/β and peroxisome proliferator-activated receptor (PPAR)-γ mRNA was examined by reverse transcription-polymerase chain reaction from inflamed and non-inflamed intestinal samples. Results: The incidence of enterocolitis increased with the volume of milk, and 50% of rat pups showed enterocolitis with a volume of 0.15 ml of milk. Expression of IĸB-α/β and PPAR-γ mRNA increased in inflamed intestine. Conclusions: Increased expression of IĸB-α/β suggested that the inflammatory mediator nuclear factor-ĸB is deeply involved in the pathogenesis of enterocolitis that can be easily introduced by overfeeding of milk ingestion in premature rat pups which mimic those seen in NEC. Increased expression of PPAR-γ may possibly regulate further development of enterocolitis in this system. |
| Databáze: | OpenAIRE |
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