Antiophidic activity of the secondary metabolite lupeol isolated from Zanthoxylum monogynum
Autor: | Mirian Machado Mendes, Klaus Casaro Saturnino, Mario Hiroyuki Hirata, Benedito Matheus dos Santos, Glaucio Monteiro Ferreira, Vanderlúcia Fonseca de Paula, Andreimar M. Soares, Maurício Temotheo Tavares, Júlio César De Bona |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
Zanthoxylum Metabolite Venom Pharmacology Toxicology complex mixtures 01 natural sciences Bothrops moojeni ANTÍDOTOS 03 medical and health sciences chemistry.chemical_compound Mice Crotalid Venoms Animals Bothrops Lupeol 0303 health sciences biology 010604 marine biology & hydrobiology 030302 biochemistry & molecular biology South America biology.organism_classification Bothrops alternatus chemistry Snake venom Pentacyclic Triterpenes |
Zdroj: | Mendes, M.M. Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
ISSN: | 1879-3150 |
Popis: | Previous studies have demonstrated the potential antiophidic activity of Zanthoxylum monogynum A.St.-Hil. a tree from the Rutaceae family native to South America. In this present contribution, we demonstrate the activity of the metabolite lupeol, a triterpenoid isolated from the stem bark of Z. monogynum against the harmful effects of the Bothrops alternatus venom. We investigated the antiophidic properties of lupeol, for this purpose, and use crude venom (Pb) incubated with lupeol in different concentrations, testing in vitro experiments and inoculated in mice for inhibitory evaluations in vivo. Besides, we tried to elucidate through the molecular dynamics the mechanism of action of lupeol with the bothropic thrombin-like toxin Jararacussin-I; the acidic phospholipase A2 toxin BthA-I from Bothrops jararacussu and the metalloproteinase toxin BmooMP-I from Bothrops moojeni. In our results, we demonstrated the potential inhibitory effect upon coagulant, phospholipasic and myotoxic activities of the bothropic venom, previously incubated with lupeol. We found that lupeol triterpenoid was able to partially inhibit local and systemic damage caused by snake venom toxins. Our in silico results demonstrate that lupeol is capable of interacting and altering the activity of the thrombin-like toxin Jararacussin-I, and capable of interacting with the BthA-I acidic PLA2, both toxins present in Bothrops snakes venom, thus demonstrating the pharmacological potential of this compound for the treatment of bothropic accidents. |
Databáze: | OpenAIRE |
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