Vasodilation and blood pressure-lowering effect mediated by 5,6-EEQ lactone in 5/6 nephrectomy hypertensive rats
| Autor: | Offir Ertracht, Tal Reuveni, Andrea Szuchman-Sapir, Michal Barsheshet, Alexandra Boginya, Shaul Atar |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Renal function Blood Pressure Vasodilation Kidney Nitric Oxide Nephrectomy Biological Factors Lactones chemistry.chemical_compound Internal medicine medicine Animals Humans Molecular Biology Microvessel Mesenteric arteries Rats Inbred Dahl Chemistry Cell Biology Eicosapentaenoic acid Rats Disease Models Animal Endocrinology Blood pressure medicine.anatomical_structure Eicosapentaenoic Acid Hypertension Epoxy Compounds Arachidonic acid Myograph |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1866:159031 |
| ISSN: | 1388-1981 |
| DOI: | 10.1016/j.bbalip.2021.159031 |
| Popis: | Microvascular dysfunction is a key contributor to vascular hypertension, one of the most common chronic diseases in the world. Microvascular dysfunction leads to the loss of nitric oxide-mediated endothelial dilation and the subsequent compensatory function of endothelium-derived hyperpolarizing (EDH) factors in the regulation of vascular tone. Previously, we showed that lactone metabolite derived from arachidonic acid induces endothelial-dependent vasodilation in isolated human microvessels. Based on structural similarities, we hypothesize that additional lactone metabolites formed from eicosapentaenoic fatty acid (EPA) may bear EDH properties. Aim To elucidate the vasodilatory and blood pressure (BP)-reducing characteristics of the 5,6-EEQ (5,6-epoxyeicosatetraenoic acids) lactone (EPA-L) in hypertensive 5/6 nephrectomy (5/6Nx) rats. Methods 5/6Nx hypertensive rats intravenously administrated with EPA-L for five days. BP, blood and urine chemistry, and kidney function were detected and analyzed. Vascular dilation was detected using a pressure myograph with or without Ca2+ − activated K+ (KCa) endothelial channel inhibitors. KCNN3 and KCNN4 gene expression (mRNA) detected in mesenteric arteries from 5/6Nx and NT rats. Results EPA-L administration to 5/6Nx rats significantly (p Conclusion EPA-L reduces BP by improving microvessel dilation involving calcium-dependent potassium endothelial channels. |
| Databáze: | OpenAIRE |
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