Antibacterial activity of gold-titanates on Gram-positive cariogenic bacteria
| Autor: | Kwok-Hung Chung, Trinuch Eiampongpaiboon, Whasun O Chung, James D. Bryers, Daniel C N Chan |
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| Rok vydání: | 2015 |
| Předmět: |
Colony-forming unit
Lactobacillus casei Materials science electron microscopy biology biology.organism_classification Streptococcus mutans Article Bacterial cell structure Microbiology Staining Biomaterials Original Article General Materials Science Antibacterial activity Bacteria caries Antibacterial agent |
| Zdroj: | Acta Biomaterialia Odontologica Scandinavica |
| ISSN: | 2333-7931 |
| DOI: | 10.3109/23337931.2015.1084883 |
| Popis: | Objective: Gram-positive cariogenic bacteria are etiological agents in dental caries; therefore, strategies to inhibit these bacteria to reduce the incident of this disease have intensified. In this study, we investigated antibacterial activities of titanates and gold-titanates against Lactobacillus casei (Lc) and Streptococcus mutans (Sm). Materials and methods: Monosodium titanate (MST), nanomonosodium titanate (nMST) and amorphous peroxo-titanate (APT), which are inorganic compounds with high-binding affinity for specific metal ions, were used. Total bacterial proteins were measured to represent bacterial cell mass after 24 h incubation with gold-titanates. We further examined the effect of nMST-Au(III) concentrations (10,200,400 mg/L) on Lc and Sm cell viability over time via Live/Dead fluorescent staining and colony forming units (CFUs). Transmission electron microscopy (TEM) was used to determine specific locations on the bacterial cells affected by the nMST-Au(III). Results: We found all gold-titanates and APT alone reduced bacterial protein for Lc (p value |
| Databáze: | OpenAIRE |
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