Transcriptome Analysis in Renal Transplant Biopsies Not Fulfilling Rejection Criteria

Autor: Daniel Serón, Joana Sellarés, Francesc Moreso, María José Soler
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Graft Rejection
Pathology
Microarrays
Review
030230 surgery
Transcriptome
lcsh:Chemistry
0302 clinical medicine
Fibrosis
microarrays
lcsh:QH301-705.5
Spectroscopy
biology
General Medicine
renal transplantation
Borderline changes
Allografts
Computer Science Applications
borderline changes
medicine.anatomical_structure
medicine.symptom
Antibody
interstitial fibrosis and tubular atrophy
medicine.medical_specialty
Tubular atrophy
Inflammation
Catalysis
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Humans
Clinical significance
Physical and Theoretical Chemistry
Molecular Biology
B cell
Biopsies
Interstitial fibrosis and tubular atrophy
business.industry
Organic Chemistry
Histology
Renal transplantation
biopsies
medicine.disease
Kidney Transplantation
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
business
transcriptome
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 6, p 2245 (2020)
International Journal of Molecular Sciences
ISSN: 1422-0067
Popis: The clinical significance of renal transplant biopsies displaying borderline changes suspicious for T-cell mediated rejection (TCMR) or interstitial fibrosis and tubular atrophy (IFTA) with interstitial inflammation has not been well defined. Molecular profiling to evaluate renal transplant biopsies using microarrays has been shown to be an objective measurement that adds precision to conventional histology. We review the contribution of transcriptomic analysis in surveillance and indication biopsies with borderline changes and IFTA associated with variable degrees of inflammation. Transcriptome analysis applied to biopsies with borderline changes allows to distinguish patients with rejection from those in whom mild inflammation mainly represents a response to injury. Biopsies with IFTA and inflammation occurring in unscarred tissue display a molecular pattern similar to TCMR while biopsies with IFTA and inflammation in scarred tissue, apart from T-cell activation, also express B cell, immunoglobulin and mast cell-related genes. Additionally, patients at risk for IFTA progression can be identified by genes mainly reflecting fibroblast dysregulation and immune activation. At present, it is not well established whether the expression of rejection gene transcripts in patients with fibrosis and inflammation is the consequence of an alloimmune response, tissue damage or a combination of both.
Databáze: OpenAIRE
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