Protein kinase C subspecies in estrogen receptor-positive and -negative human breast cancer cell lines
| Autor: | Kouji Ogita, Akira Kishimoto, Yasutomi Nishizuka, Eric Bignon |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Biophysics Estrogen receptor Breast Neoplasms Biology Biochemistry Isozyme Cell Line Enzyme activator Internal medicine Biomarkers Tumor medicine Humans Receptor Molecular Biology Protein Kinase C Protein kinase C Diacylglycerol kinase Cell Biology Chromatography Ion Exchange Molecular biology Enzyme Activation Isoenzymes Kinetics Endocrinology Receptors Estrogen Estrogen Cell culture Tetradecanoylphorbol Acetate Calcium Female Subcellular Fractions |
| Zdroj: | Biochemical and Biophysical Research Communications. 171:1071-1078 |
| ISSN: | 0006-291X |
| Popis: | Estrogen receptor-positive (MCF7) and -negative (BT20) human breast cancer cell lines, which are frequently used for studies on cancer chemotherapy with triphenylethylene (TPE) anti-estrogens, express at least three protein kinase C subspecies. Two of them are identified as type II PKC having the beta-sequence and type III PKC having the alpha-sequence. The other one shows typical characteristics of PKC which responds to Ca2+, phosphatidylserine and diacylglycerol, but shows kinetic properties subtly different from the previously known PKC subspecies. Immunoblot analysis has shown that this enzyme does not correspond to any of the well defined subspecies with known sequence structures. All of these PKC subspecies are similarly susceptible to the TPE antiestrogens. |
| Databáze: | OpenAIRE |
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