Cycle-dependent matrix remodeling gene expression response in fatigue-loaded rat patellar tendons
| Autor: | Vincent M. Wang, Jonathan P. Braman, Jelena Basta-Pljakic, Jonathan Y. Lee, Daniel J. Leong, Mitch B. Schaffler, Hui B. Sun, Yonghui Li, David T. Fung, Raymond A. Klug, Evan L. Flatow, Nelly Andarawis-Puri, Jedd B. Sereysky, Stephen J. Ros, Karl J. Jepsen |
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| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Integrin Bone Matrix Matrix metalloproteinase Article Bone remodeling Rats Sprague-Dawley Weight-Bearing Downregulation and upregulation Patellar Ligament Gene expression medicine Animals Orthopedics and Sports Medicine Fatigue Regulation of gene expression biology Chemistry Patellar ligament Tissue Inhibitor of Metalloproteinases Matrix Metalloproteinases Rats Cell biology Tendon medicine.anatomical_structure Gene Expression Regulation Models Animal biology.protein Female Bone Remodeling Collagen |
| Zdroj: | Journal of Orthopaedic Research. 28:1380-1386 |
| ISSN: | 0736-0266 |
| Popis: | Expression profiling of selected matrix remodeling genes was conducted to evaluate differences in molecular response to low-cycle (100) and high-cycle (7,200) sub-failure-fatigue loading of patellar tendons. Using our previously developed in vivo patellar tendon model, tendons were loaded for 100 or 7,200 cycles and expression of selected metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and collagens were quantified by real-time RT-PCR at 1- and 7-day post-loading. Expression profiles were also obtained from lacerated tendons as an acute injury model. The high-cycle group showed upregulation of TIMP-1, -2, Col3a1, and Col5a1, and downregulation TIMP-4 at both time points, upregulation of MMP-2 at 7-day post-loading and downregulation of MMP-13 and -14 at 1-day post-loading, suggesting overall repair/remodeling. In contrast, the low-cycle loaded group showed upregulation of MMP-2, -3, -13, and Col12a1 at both time points, upregulation of TIMP-1, -2, -3, Col3a1, and integrin β1 and downregulation of integrin α11 at 1-day post-loading and upregulation of Col1a1 at 7-day post-loading, consistent with a hypertrophic (adaptive) pattern. Lacerated tendons showed a typical acute wound response with upregulation of all examined remodeling genes. Differences found in tendon response to high- and low-cycle loading are suggestive of the underlying mechanisms associated with a healthy or damaging response. |
| Databáze: | OpenAIRE |
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