Micronucleus test and comet assay for the evaluation of zebrafish genomic damage induced by erythromycin and lincomycin
Autor: | Carmela Peluso, Vincenzo Stingo, Lucia Rocco |
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Přispěvatelé: | Rocco, Lucia, C., Peluso, Stingo, Vincenzo |
Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
Male
Erythrocytes DNA damage medicine.drug_class Health Toxicology and Mutagenesis Antibiotics Erythromycin Management Monitoring Policy and Law Biology Pharmacology Toxicology medicine.disease_cause Micronucleus test medicine Animals Zebrafish Comet assay Genetics Micronucleus Tests General Medicine biology.organism_classification Lincomycin Hepatocytes Female Genotoxicity Water Pollutants Chemical medicine.drug DNA Damage |
Popis: | An enormous quantity of pharmacologically active principles are currently being introduced into the environment, with consequent escalation of environmental problems, but only a small number of studies are focusing on an assessment of their genotoxic effects. The aim of this article is to assess the genotoxic effects of erythromycin, lincomycin, and of a combination of these two antibiotics on the genome of the zebrafish. The genotoxicity of the two antibiotics was assessed by applying the micronucleus test to erythrocytes and performing a Comet assay on erythrocytes and hepatocytes. The fish were exposed to antibiotics at different concentrations and times of exposure, under standard laboratory conditions. Depending on the different experimental conditions, erythromycin and lincomycin induced a significant increase in DNA migration (tail moment) and a significant increase in micronuleus frequency. We also conducted an analysis on the activation of repair mechanisms when the genotoxic agent was removed. Only a few of the cells displayed a decrease in damage under these test conditions. © 2011 Wiley Periodicals, Inc. |
Databáze: | OpenAIRE |
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