Association of HBsAg mutation patterns with hepatitis B infection outcome: Asymptomatic carriers versus HCC/cirrhotic patients

Autor: Neda Sanaei, Jamal Sarvari, Seyed A. Malek-Hosseini, Mohamad-Reza Fattahi, Seyed Younus Hosseini
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Serotype
Liver Cirrhosis
Male
HBsAg
Epitopes
T-Lymphocyte
Specialties of internal medicine
medicine.disease_cause
Gastroenterology
Epitope
0302 clinical medicine
Genotype
Medicine
Asymptomatic Infections
Liver Neoplasms
General Medicine
Middle Aged
RC581-951
030220 oncology & carcinogenesis
Carrier State
Epitopes
B-Lymphocyte
030211 gastroenterology & hepatology
Female
medicine.symptom
Adult
medicine.medical_specialty
Hepatitis B virus
Carcinoma
Hepatocellular
Gene Products
pol
Asymptomatic
03 medical and health sciences
Young Adult
Hepatitis B
Chronic
Antigen
Internal medicine
Hepatitis B surface antigen (HBsAg)
Drug Resistance
Viral
Humans
Immune Evasion
Sequence variation
Disease progression
Hepatitis B Surface Antigens
Hepatology
business.industry
digestive system diseases
DNA
Viral
Mutation
business
Asymptomatic carrier
Zdroj: Annals of Hepatology, Vol 18, Iss 4, Pp 640-645 (2019)
ISSN: 1665-2681
Popis: Introduction and Objectives The hepatitis B virus (HBV) surface antigen (HBsAg) variations suggested having some effects on infection outcome. Due to some controversial issues, the aim of this study was to compare the pattern of HBsAg variation between asymptomatic carriers and HCC/cirrhosis patients. Materials and methods In this cross-sectional study, 19 HCC/cirrhotic and 26 asymptomatic patients were enrolled. After viral DNA extraction, HBs gene was amplified using an in-house nested-PCR. Then, PCR products were introduced into bi-directional Sanger sequencing. The retrieved sequences were compared with references, to investigate the variation of immunologic sites, major hydrophilic region (MHR) of HBsAg as well as reverse transcriptase (RT), and also to determine genotype/subtype. Results The analysis of MHR and epitopes on HBsAg showed dozens of substitution, which occurred more prevalently in I110, P120, Y134, G159, S193, Y206, S207, I208, L213 and P214 positions. However, Y134N/F/L (P = 0.04) and P120T/S (P = 0.009) were significantly detected in MHR and B-cell epitope of HCC/Cirrhotic group. A number of truncation-related mutations were higher in HCC/Cirrhotic group (P > 0.001), albeit only C69* stop codon was statistically significant (P = 0.003). In RT, some potentially resistant substitutions such as Q215S, V191I and V214A, were revealed. Phylogenetic analysis showed that all of isolates belonged to genotype D, and the major serotype was ayw1. Conclusion The higher frequency of substitutions in MHR and immune epitopes at positions such as Y134 and P120 as well as stop codons such as C69* in HCC/cirrhotic group might candidate them as predictive factors for infection outcome.
Databáze: OpenAIRE
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