Flow-Cytometric Characterization of Hematopoietic Cells in Non-Pregnant Human Endometrium
| Autor: | Pierre Miron, Robert Hemmings, Denis-Claude Roy, M.-H. Lachapelle, Chantal Baron |
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| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Immunology Cell Separation Biology Endometrium Immunophenotyping Flow cytometry Andrology Leukocyte Count chemistry.chemical_compound Antigen Leukocytes medicine Humans Immunology and Allergy Tissue Distribution Propidium iodide CD20 medicine.diagnostic_test Reproducibility of Results Obstetrics and Gynecology Flow Cytometry Killer Cells Natural medicine.anatomical_structure Reproductive Medicine chemistry biology.protein Female CD8 Endometrial biopsy |
| Zdroj: | American Journal of Reproductive Immunology. 35:5-13 |
| ISSN: | 1046-7408 |
| DOI: | 10.1111/j.1600-0897.1996.tb00002.x |
| Popis: | Lachapelle M-H, Miron P, Hemmings R, Baron C, Roy DC. Flow-cytometric characterization of hematopoietic cells in non-pregnant human endometrium. Am J Reprod Immunol 1996; 35:5–13 cP Munksgaard, Copenhagen PROBLEM: Immunologic evaluation and quantitation of hematopoietic cells in human endometrium has been difficult to perform, particularly in nonpregnant subjects. In this study, we describe a method for the flow-cytometric characterization of hematopoietic cells present in the endometrium of non-pregnant women. METHOD: Endometrial biopsy samples from normal donors were first mechanically disrupted and filtered to generate a single-cell suspension of leukocyte-enriched endometrial cells. Cells were labeled with a panel of monoclonal antibodies, stained with propidium iodide (PI), and one- or two-color flow-cytometric analysis performed on cells excluding PI. RESULTS: The methodology described in this study was highly reproducible in experiments evaluating the interrun and intrarun variability. We then determined the immunophenotypic profile of endometrial leukocytes from 12 normal females. The majority of leukocytes were T cells (CD3: 47%; CD4: 24%; CD8: 28%) with an important contingent of NK cells (CD56: 32%), the majority of which harbored the unusual CD16-CD56 bright phenotype, and a minority of B cells (CD20: 6%) and monocytes (CD14: 7%). CONCLUSIONS: Flow cytometry can be used to assess antigen expression on the surface of endometrial leukocytes from nonpregnant women. In future studies, it will be possible to use this approach to investigate the role of immune cell populations in the endometrium of patient experiencing reproductive failure. |
| Databáze: | OpenAIRE |
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