Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae
| Autor: | Ami Miyata, Hiroki Toyoshima, Shinichi Takaichi, Risako Yoshida, Taichiro Ishige, Shinji Kawasaki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
carotenoprotein Light QH301-705.5 Pharmaceutical Science Chlamydomonas reinhardtii Xanthophylls 01 natural sciences Coelastrella Article Scenedesmus 03 medical and health sciences chemistry.chemical_compound astaxanthin-binding protein Astaxanthin Chlorophyta Drug Discovery Scenedesmaceae Biology (General) Pharmacology Toxicology and Pharmaceutics (miscellaneous) Gene Peptide sequence 030304 developmental biology 0303 health sciences biology Chemistry Water ASTAP biology.organism_classification astaxanthin Oxidative Stress Biochemistry Solubility Carrier Proteins 010606 plant biology & botany |
| Zdroj: | Marine Drugs Volume 19 Issue 6 Marine Drugs, Vol 19, Iss 349, p 349 (2021) |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md19060349 |
| Popis: | Photooxidative stress-inducible water-soluble astaxanthin-binding proteins, designated as AstaP, were identified in two Scenedesmaceae strains, Coelastrella astaxanthina Ki-4 and Scenedesmus obtusus Oki-4N both strains were isolated under high light conditions. These AstaPs are classified as a novel family of carotenoprotein and are useful for providing valuable astaxanthin in water-soluble form however, the distribution of AstaP orthologs in other microalgae remains unknown. Here, we examined the distribution of AstaP orthologs in the family Scenedesmaceae with two model microalgae, Chlamydomonas reinhardtii and Chlorella variabilis. The expression of AstaP orthologs under photooxidative stress conditions was detected in cell extracts of Scenedesmaceae strains, but not in model algal strains. Aqueous orange proteins produced by Scenedesmaceae strains were shown to bind astaxanthin. The protein from Scenedesmus costatus SAG 46.88 was purified. It was named ScosAstaP and found to bind astaxanthin. The deduced amino acid sequence from a gene encoding ScosAstaP showed 62% identity to Ki-4 AstaP. The expression of the genes encoding AstaP orthologs was shown to be inducible under photooxidative stress conditions however, the production amounts of AstaP orthologs were estimated to be approximately 5 to 10 times lower than that of Ki-4 and Oki-4N. |
| Databáze: | OpenAIRE |
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