Non-invasive Screening for Treatment of Heifers with the Anabolic Steroid Melengestrol Acetate (MGA) by Feces Analysis

Autor:	Andreas Daxenberger, M. Hageleit, Michael W. Pfaffl, Heinrich H. D. Meyer, Iris G. Lange
Rok vydání:	2003
Předmět:	Anabolism medicine.medical_treatment Clinical Biochemistry Immunology Administration Oral Enzyme-Linked Immunosorbent Assay Food Contamination Urine Melengestrol acetate Feces chemistry.chemical_compound Anabolic Agents medicine Animals Immunology and Allergy Melengestrol Acetate Chromatography medicine.diagnostic_test Feces analysis Non invasive Medical Laboratory Technology chemistry Immunoassay Cattle Female Anabolic steroid
Zdroj:	Journal of Immunoassay and Immunochemistry. 24:265-272
ISSN:	1532-4230 1532-1819
DOI:	10.1081/ias-120022936
Popis:	For eight weeks, two heifers each had been orally administered daily doses of 0, 1.5, or 5 mg melengestrol acetate (MGA) in a feed premix. Four heifers received the labeled dose of 0.5 mg/day. Regular feces samples were taken throughout the experiment. A rapid screening method for the determination of MGA in feces was developed, consisting of liquid extraction, clean-up on solid-phase extraction cartridges and quantification by enzyme immunoassay (ELISA). Residues in feces were dose-dependent with mean values of
Databáze:	OpenAIRE
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