The Up-Regulated Expression of Tenascin C in Human Nasal Polyp Tissues is Related to Eosinophil-Derived Transforming Growth Factor β1
| Autor: | Qixue Gao, Heng Wang, Xiang Lu, Yonghua Cui, Zheng Liu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Adolescent Transforming Growth Factor beta1 03 medical and health sciences Nasal Polyps 0302 clinical medicine Downregulation and upregulation medicine Humans Nasal polyps RNA Messenger Child 030223 otorhinolaryngology Cells Cultured biology Reverse Transcriptase Polymerase Chain Reaction business.industry Tenascin C Epithelial Cells Tenascin Middle Aged respiratory system Eosinophil medicine.disease Immunohistochemistry Up-Regulation Cell biology Eosinophils Nasal Mucosa medicine.anatomical_structure Otorhinolaryngology Cell culture Transforming growth factor beta 3 030220 oncology & carcinogenesis biology.protein Female business Transforming growth factor |
| Zdroj: | American Journal of Rhinology. 20:629-633 |
| ISSN: | 1539-6290 1050-6586 |
| DOI: | 10.2500/ajr.2006.20.2918 |
| Popis: | Background Tissue remodeling is an important characteristic of nasal polyps (NPs). However, the mechanisms underlying the remodeling processes are poorly defined. This study investigated the role of transforming growth factor (TGF) β1 and eosinophils in the expression of tenascin C (Tn-C), an extracellular matrix glycoprotein, in NPs. Methods The protein expression of Tn-C and TGF-β1 was examined by means of immunohistochemistry in NPs and normal control inferior turbinate tissues. Furthermore, cell culture, quantitative RT-PCR, and in situ immunocytofluorescence techniques were used to investigate the direct effect of TGF-β1 and eosinophils on Tn-C production in primary nasal epithelial cells. Results Tn-C protein expression was significantly up-regulated in NP tissues and correlated with TGF-β1+ eosinophils. TGF-β1 and eosinophils dramatically induced Tn-C mRNA and protein expression in nasal epithelial cells. The effect of eosinophils could be inhibited partly by a neutralizing antibody to TGF-β1. Conclusion Eosinophil-derived TGF-β1 may contribute, at least in part, to the tissue remodeling in NPs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|