Anti-inflammatory activity of Protium spruceanum (Benth.) Engler is associated to immunomodulation and enzymes inhibition
| Autor: | Fernando Augusto Siqueira Mathias, João Filipe Pereira Vieira, Vivette Appolinário Rodrigues Cabral, Tatiane Roquete Amparo, Geraldo Célio Brandão, Paula Melo de Abreu Vieira, Janaína Brandão Seibert, Orlando David Henrique dos Santos, Kátia Michelle Freitas, Rodrigo Dian de Oliveira Aguiar Soares, Gustavo Henrique Bianco de Souza |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.drug_class
Anti-Inflammatory Agents Nitric Oxide Synthase Type II Pharmacology Nitric Oxide Anti-inflammatory Cell Line Immunomodulation Mice 03 medical and health sciences chemistry.chemical_compound Rutin 0302 clinical medicine Western blot Acetylglucosaminidase Drug Discovery medicine Animals Zymography 030304 developmental biology 0303 health sciences Plant Stems medicine.diagnostic_test biology Plant Extracts Catechin Quercitrin Plant Leaves Nitric oxide synthase Matrix Metalloproteinase 9 chemistry Cyclooxygenase 2 030220 oncology & carcinogenesis biology.protein Cytokines Cyclooxygenase Reactive Oxygen Species Burseraceae |
| Zdroj: | Journal of Ethnopharmacology. 241:112024 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2019.112024 |
| Popis: | Ethnopharmacological relevance Protium spruceanum (Burseraceae) is used in Brazilian traditional medicine as anti-inflammatory, but the factors involved in this activity were not yet characterized. Aims of the study: analyze the aspects involved in the anti-inflammatory activity of polar fractions obtained from extracts of leaves and branches. Materials and methods: Hydromethanolic fraction was obtained by liquid-liquid partition from crude ethanolic extract and its compounds were identified by LC-DAD-MS. Activity tests were performed using LPS + IFN-γ stimulated J774A.1 macrophages. Cytokines were evaluated by CBA kit, NO by Griess method, ROS by DCFH-DA, N-acetylglucosaminidase (NAG) activity by spectrophotometric method, matrix-metalloproteinase (MMP-9) activity by zymography, inducible nitric oxide synthase (iNOS) expression by immunofluorescence and cyclooxygenase (COX-2) expression by Western blot. Results: Fractions induced an increase of IL-6 and IL-10 which leads to the control of pro-inflammatory cytokines levels. The treatment with the fractions also reduced NO production at all concentrations tested in all evaluated periods. ROS production by the macrophages was inhibited by the treatment and the leaves fraction showed the best results with a lower concentration than that observed for the branches. The enzymes assays showed that leaves fraction inhibited NAG and MMP-9 activities, as well as, iNOS and COX-2 expression. These activities can be associated with the presence of procyanidin, catechin, rutin, quercitrin, isoquercitrin and kaempferol-3-O-rhamnoside, major compounds that were identified in the fraction. Conclusions: Anti-inflammatory activity of P. spruceanum is associated to an immunomodulatory effect that leads to inhibition of ROS, NO, NAG, MMP-9, COX-2 and iNOS. |
| Databáze: | OpenAIRE |
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