Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing
| Autor: | Marina Falaleeva, Zaneta Matuszek, Amadís Pagès, Ruth Sperling, Yuval Nevo, Stefan Stamm, Lily Agranat-Tamir, Eduardo Eyras, Konstantin V. Korotkov, Sana Hidmi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromosomal Proteins Non-Histone Molecular Sequence Data Biology Cell Fractionation Methylation Ribonucleoprotein U1 Small Nuclear 03 medical and health sciences RRNA modification E2F7 Transcription Factor RNA Precursors Humans RNA Small Nucleolar Small nucleolar RNA RNA Processing Post-Transcriptional Base Pairing Ribonucleoprotein Genetics Fibrillarin Cell Nucleus Multidisciplinary Organelle Biogenesis Base Sequence Alternative splicing Cell Cycle RNA Exons Oligonucleotides Antisense Cell biology Alternative Splicing 030104 developmental biology Solubility PNAS Plus RNA Ribosomal Gene Knockdown Techniques RNA splicing Spliceosomes Ribosomes Small nuclear ribonucleoprotein Cell Division HeLa Cells |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 113(12) |
| ISSN: | 1091-6490 |
| Popis: | Significance C/D box small nucleolar RNAs (SNORDs) are abundant, short, nucleoli-residing, noncoding RNAs that guide the methyltransferase fibrillarin to perform 2′- O -methylation of target RNAs. We identified 29 SNORDs present in a fibrillarin-containing fraction as well as a fibrillarin-free fraction enriched in spliceosomes. One of these SNORDs, SNORD27 , directs rRNA methylation and regulates alternative pre-mRNA splicing (AS) of E2F7 pre-mRNA, a transcriptional repressor of cell cycle-regulated genes. SNORD27 likely regulates E2F7 pre-mRNA AS by masking splice sites through base pairing. This previously unidentified function of SNORDs increases the number of factors regulating AS, a critical step in the expression of the vast majority of human genes, and highlights a potential coupling between AS, cell cycle, proliferation, and ribosome biogenesis. |
| Databáze: | OpenAIRE |
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