Characterization of in vitro phase I metabolites of methamnetamine in human liver microsomes by liquid chromatography-quadrupole time-of-flight mass spectrometry
| Autor: | Hye Hyun Yoo, Mi Sun Kang, Jin-Moo Lee, Sun-Ok Choi, Young-ki Hong, Young-Hoon Kim |
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| Rok vydání: | 2020 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Chromatography Metabolite Metabolism Monooxygenase In Vitro Techniques Tandem mass spectrometry Mass spectrometry Hydroxylation Pathology and Forensic Medicine Demethylation Substance Abuse Detection chemistry.chemical_compound chemistry Tandem Mass Spectrometry Microsome Microsomes Liver Oxygenases Humans Drug metabolism Chromatography Liquid |
| Zdroj: | International journal of legal medicine. 135(4) |
| ISSN: | 1437-1596 |
| Popis: | N-Methyl-1-(naphthalen-2-yl)propan-2-amine (methamnetamine, PAL-1046) is an amphetamine-based new psychoactive substance (NPS). Methamnetamine has been reported to cause excessive release of serotonin, and it is classified as an empathogen or entactogen. It is not regulated as a controlled substance in most countries, and there are no studies on its metabolism. In this study, in vitro phase I metabolism of methamnetamine in human liver microsomes (HLM) and flavin-containing monooxygenase (FMO) was investigated by liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS). Eight metabolites of methamnetamine were identified and were structurally characterized achieved by a combination of accurate mass analysis and tandem mass spectrometry. The identified metabolic processes include N-demethylation, N-hydroxylation, aromatic hydroxylation, and a combination of these processes. N-Hydroxylated metabolites were confirmed based on expressed FMOs. The major metabolite was formed from methamnetamine via hydroxylation of the naphthalene ring after the in vitro phase I process. These results could help detect methamnetamine ingestion by NPS abusers. |
| Databáze: | OpenAIRE |
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