Impact of human visceral and glutealfemoral adipose tissue transplant on glycemic control in a mouse model of diet-induced obesity
| Autor: | Renea A. Taylor, Paul Burton, Matthew J. Watt, Thomas Tsiloulis, Stacey N Keenan, Geraldine J. Ooi, Arthe Raajendiran |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Blood Glucose Male medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Subcutaneous Fat Adipokine Adipose tissue Neovascularization Physiologic 030209 endocrinology & metabolism Glycemic Control Carbohydrate metabolism Intra-Abdominal Fat Diet High-Fat 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Insulin resistance Physiology (medical) Internal medicine Adipocyte Medicine Glucose homeostasis Animals Homeostasis Humans Obesity Triglyceride business.industry Insulin Middle Aged medicine.disease Lipid Metabolism 030104 developmental biology Endocrinology chemistry Adipose Tissue Body Composition Female Collagen Insulin Resistance business Energy Metabolism |
| Zdroj: | American journal of physiology. Endocrinology and metabolism. 319(3) |
| ISSN: | 1522-1555 |
| Popis: | Regional distribution of adipose tissue is an important factor in conferring cardiometabolic risk and obesity-related morbidity. We tested the hypothesis that human visceral adipose tissue (VAT) impairs glucose homeostasis, whereas subcutaneous glutealfemoral adipose tissue (GFAT) protects against the development of impaired glucose homeostasis in mice. VAT and GFAT were collected from patients undergoing bariatric surgery and grafted onto the epididymal adipose tissue of weight- and age-matched severe, combined immunodeficient mice. SHAM mice underwent surgery without transplant of tissue. Mice were fed a high-fat diet after xenograft. Energy homeostasis, glucose metabolism, and insulin sensitivity were assessed 6 wk later. Xenograft of human adipose tissues was successful, as determined by histology, immunohistochemical evaluation of collagen deposition and angiogenesis, and maintenance of lipolytic function. Adipose tissue transplant did not affect energy expenditure, food intake, whole body substrate partitioning, or plasma free fatty acid, triglyceride, and insulin levels. Fasting blood glucose was significantly reduced in GFAT and VAT compared with SHAM, whereas glucose tolerance was improved only in mice transplanted with VAT compared with SHAM mice. This improvement was not associated with differences in whole body insulin sensitivity or plasma insulin between groups. Together, these data suggest that VAT improves glycemic control and GFAT does not protect against the development of high-fat diet-induced glucose intolerance. Hence, the intrinsic properties of VAT and GFAT do not necessarily explain the postulated negative and positive effects of these adipose tissue depots on metabolic health. |
| Databáze: | OpenAIRE |
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