Simultaneous determination of diclofenac and its common counter-ions in less than 1 minute using capillary electrophoresis with contactless conductivity detection
| Autor: | Denise Tofanello Gimenes, Rafael R. Cunha, Claudimir Lucio do Lago, Rodrigo A.A. Munoz, Eduardo M. Richter |
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| Rok vydání: | 2013 |
| Předmět: |
ELETROFORESE (MÉTODO DE SEPARAÇÃO)
Diclofenac Time Factors Sodium Potassium Clinical Biochemistry Analytical chemistry chemistry.chemical_element Electrolyte Biochemistry High-performance liquid chromatography Analytical Chemistry Capillary electrophoresis Drug Stability Limit of Detection Ions Detection limit Active ingredient Chromatography Anti-Inflammatory Agents Non-Steroidal Electric Conductivity CROMATOGRAFIA LÍQUIDA DE ALTA EFICIÊNCIA Electrophoresis Capillary Quaternary Ammonium Compounds Electrophoresis chemistry Tablets |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0173-0835 |
| Popis: | This paper presents a method for fast and simultaneous determination of diclofenac (DCF) and its common counter-ions (potassium, sodium, and diethylammonium) using CE with capacitively coupled contactless conductivity detection (CE-C(4) D). On the basis of a single electropherogram (about 50 s), the proposed method allows the determination of the stoichiometry, absolute quantification and evaluation of the degradation degree of the active pharmaceutical ingredient (DCF). A linear working range from 100 to 500 μmol/L was obtained for all analytes in an equimolar TRIS/TAPS (10 mmol/L) solution as the background electrolyte as well as adequate LOD (7, 6, 7, and 10 μmol/L for K(+) , Na(+) , diethylammonium, and DCF, respectively). The proposed method was applied to the analysis of pharmaceutical formulations (tablets and spray form) with similar results to those achieved by HPLC (DCF) or flame photometry (K and Na) at a 95% confidence level. |
| Databáze: | OpenAIRE |
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