Inhibition of splicing and nuclear retention of pre-mRNA by spliceostatin A in fission yeast
| Autor: | Akihisa Matsuyama, Shinichi Nishimura, Chor Wai Lo, Sueharu Horinouchi, Daisuke Kaida, Hidenori Nakajima, Minoru Yoshida, Hiroshi Taoka, Yoko Yashiroda, Hidenori Watanabe, Tokio Tani, Ken Ishigami |
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| Rok vydání: | 2007 |
| Předmět: |
Cell Nucleus
Spliceosome biology RNA Splicing Saccharomyces cerevisiae Biophysics Translation (biology) Cell Biology biology.organism_classification Biochemistry Yeast Fungal Proteins Saccharomyces Cytoplasm RNA splicing RNA Precursors Spliceosomes Precursor mRNA Molecular Biology Function (biology) |
| Zdroj: | Biochemical and Biophysical Research Communications. 364:573-577 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.10.029 |
| Popis: | Nuclear retention of pre-mRNAs is tightly regulated by several security mechanisms that prevent pre-mRNA export into the cytoplasm. Recently, spliceostatin A, a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. Here we report that spliceostatin A also inhibits splicing and nuclear retention of pre-mRNA in a fission yeast strain that lacks the multidrug resistance protein Pmd1. As observed in mammalian cells, spliceostatin A is bound to components of the SF3b complex in the spliceosome. Furthermore, overexpression of nup211, a homolog of Saccharomyces cerevisiae MLP1, suppresses translation of pre-mRNAs accumulated by spliceostatin A. These results suggest that the SF3b complex has a conserved role in pre-mRNA retention, which is independent of the Mlp1 function. |
| Databáze: | OpenAIRE |
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