Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal
| Autor: | Nadine Raymond, Ying Wang, Kayan Tam, Scott I. Simon, Anthony G. Passerini, Tyler M. Tomita, John Schulze |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Endothelium Physiology Vascular Biology and Microcirculation Lipoproteins medicine.medical_treatment Vascular Cell Adhesion Molecule-1 Inflammation Biology Monocytes Young Adult chemistry.chemical_compound Physiology (medical) Internal medicine E-selectin medicine Humans Aorta Cells Cultured Triglycerides Triglyceride Tumor Necrosis Factor-alpha Endothelial Cells Intercellular Adhesion Molecule-1 Postprandial Period Dietary Fats Endothelial stem cell Cytokine Endocrinology Postprandial medicine.anatomical_structure chemistry Obesity Abdominal biology.protein Female Waist Circumference medicine.symptom E-Selectin Cardiology and Cardiovascular Medicine Lipoprotein |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 300:H784-H791 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.01036.2010 |
| Popis: | A rise in postprandial serum triglycerides (PP-sTG) can potentiate inflammatory responses in vascular endothelial cells (ECs) and thus serves as an independent risk factor for predicting increased cardiovascular morbidity. We examined postprandial triglyceride-rich lipoproteins (PP-TGRLs) in subjects ranging from normal to hypertriglyceridemic for their capacity to alter EC acute inflammatory responses. Cultured human aortic ECs (HAECs) were conditioned with PP-TGRLs isolated from human serum at the peak after a moderately high-fat meal. VLDL particle size increased postprandially and varied directly with the subject's PP-sTG level and waist circumference. PP-TGRL particles bound to HAECs and were internalized via LDL receptor-mediated endocytosis. PP-TGRL alone did not induce an inflammatory response over the range of individuals studied. However, combined with low-dose TNF-α stimulation (0.3 ng/ml), it elicited a net 10–15% increase above cytokine alone in the membrane expression of VCAM-1, ICAM-1, and E-selectin, which was not observed with fasting TGRLs. In contrast to upregulation of ICAM-1 and E-selectin, VCAM-1 transcription and expression varied in direct proportion with individual PP-sTG and waist circumference. The extent of monocyte arrest on inflamed HAECs under shear stress also correlated closely with VCAM-1 expression induced by conditioning with PP-TGRL and TNF-α stimulation. This ex vivo approach provides a quantitative means to assess an individual's inflammatory potential, revealing a greater propensity for endothelial inflammation in hypertriglyceridemic individuals with abdominal obesity. |
| Databáze: | OpenAIRE |
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