The precipitation of diphtheria antitoxin by means of precipitins

Autor:	J. P. Atkinson, E. J. Banzhaf
Rok vydání:	1910
Předmět:	biology Globulin Chemistry Diphtheria antitoxin Diphtheria Normal serum medicine.disease Precipitin complex mixtures General Biochemistry Genetics and Molecular Biology Microbiology Immunology medicine biology.protein Normal Horse Serum Antibody
Zdroj:	Experimental Biology and Medicine. 7:148-150
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-7-94
Popis:	This work was undertaken partly to check and to review the previous observations by one of us a number of years ago on the precipitation of diphtheria antitoxin from solutions by means of antibodies prepared by injecting diphtheria antitoxic globulin and globulin of normal horse serum into rabbits,1 partly to determine whether such a method could be used in the further purification of diphtheria antitoxin.In these earlier experiments it was found that the antibody formed by the injection of globulin of normal serum into rabbits threw out of solution diphtheria antitoxin just as well as the antibody formed by the injection of diphtheria antitoxic globulin.We undertook these last experiments with the purest diphtheria antitoxin fractionated from the globulin, and serum globulin fractionated in the same way and under the same conditions. The sera were kept at 56° C. for fifteen hours to convert, as far as possible, pseudo-globulin into eu-globulin. The diphtheria antitoxin under these conditions remains unch...
Databáze:	OpenAIRE
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