Extracellular vesicles derived from macrophages display glycyl‐tRNA synthetase 1 and exhibit anti‐cancer activity
| Autor: | Chihong Song, Peter C. Goughnour, Hyun Suk Jung, Min Chul Park, Jae Kyung Hyun, Yong Song Gho, Daehee Hwang, Byung-Gyu Kim, Sangmi Jun, Won Suk Yang, Sehyun Chae, Sunghoon Kim, Kim Sangbum, Seong Min Cho, Ji Hyun Lee |
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| Rok vydání: | 2020 |
| Předmět: |
Glycine-tRNA Ligase
0301 basic medicine Histology Carcinogenesis medicine.medical_treatment Apoptosis Vimentin macrophage Extracellular Vesicles Mice 03 medical and health sciences glycyl‐tRNA synthetase 1 0302 clinical medicine Palmitoylation Cell Line Tumor Neoplasms medicine Animals palmitoylation Secretion Receptor Research Articles biology Chemistry Macrophages Tumor Suppressor Proteins Growth factor Cell Biology Cell biology Cytosol RAW 264.7 Cells 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell biology.protein tumour microenvironment Intracellular Research Article |
| Zdroj: | Journal of Extracellular Vesicles |
| ISSN: | 2001-3078 |
| DOI: | 10.1002/jev2.12029 |
| Popis: | Glycyl‐tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13–1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1‐containing EVs (GARS1‐EVs). Among the identified factors, insulin‐like growth factor II receptor, and vimentin also contributed to the anti‐cancer activity of GARS1‐EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis. |
| Databáze: | OpenAIRE |
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