Caffeine treatment disturbs the angiogenesis of zebrafish embryos
| Autor: | Chien-Chung Cheng, Chi-Chung Wen, Yun-Feng Liao, Jen Ning Tsai, Yun-Hsin Wang, Chien-Hung Yeh, Yau-Hung Chen, Chao-Yuan Chang |
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| Rok vydání: | 2012 |
| Předmět: |
animal structures
Embryo Nonmammalian Endothelium Angiogenesis Health Toxicology and Mutagenesis Green Fluorescent Proteins Down-Regulation Neovascularization Physiologic Toxicology Real-Time Polymerase Chain Reaction Neovascularization Animals Genetically Modified Vasculogenesis Downregulation and upregulation Caffeine medicine Animals Nerve Growth Factors Zebrafish Pharmacology Chemical Health and Safety biology Dose-Response Relationship Drug Embryogenesis Public Health Environmental and Occupational Health General Medicine Zebrafish Proteins biology.organism_classification Embryonic stem cell Molecular biology Cell biology Up-Regulation medicine.anatomical_structure Teratogens embryonic structures Endothelium Vascular medicine.symptom |
| Zdroj: | Drug and chemical toxicology. 35(4) |
| ISSN: | 1525-6014 |
| Popis: | Caffeine is a widely consumed substance that occurs in numerous dietary sources, but teratogenic effects of caffeine intake during embryonic development are still not clear. In the present study, we used the zebrafish as a model to assess caffeine-induced toxicity on embryonic vascular development. A green fluorescent vascular endothelium transgenic line, Tg(fli1:egfp), was utilized for the sensitive detection of vascular development, including vasculo- and angiogenesis. Caffeine-treated embryos showed no defects in vasculogenesis, but revealed dose-dependent (250-350 ppm) developmental defects in intersegmental vessels, dorsal longitudinal anastomotic vessels, and subintestinal vein sprouting. Further, real-time polymerase chain reaction analysis of caffeine-treated embryos showed an upregulation of nrp1a along with a downregulation of sema3aa and sema3c. In conclusion, caffeine treatment induces defects of angiogenesis in zebrafish embryos. |
| Databáze: | OpenAIRE |
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