The properties of a mammary gland cancer stem cell
| Autor: | Ileana Zucchi, Rolland Reinbold, S. Sanzone, Gloria Bertoli, Ottavia Barbieri, Renato Dulbecco, Alberto Albertini, V. Valsecchi, Simonetta Astigiano, Paride Pelucchi, Maddalena Scotti |
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| Rok vydání: | 2007 |
| Předmět: |
cancer stem cells
Cellular differentiation Cell Transplantation Heterologous Carbazoles Breast Neoplasms Mice SCID Biology Adenocarcinoma single cell injection Mice Mammary Glands Animal Organ Culture Techniques stem cells Cancer stem cell Mice Inbred NOD Cell Line Tumor p21/WAF1 medicine Animals Cell Lineage Fluorescent Antibody Technique Indirect mammary gland differentiation Cells Cultured Cell Proliferation Fluorescent Dyes Multidisciplinary Keratin-18 Cell growth Keratin-14 Cell Differentiation Biological Sciences medicine.disease Immunohistochemistry Cell biology Clone Cells Rats Endothelial stem cell medicine.anatomical_structure Immunology Neoplastic Stem Cells Benzimidazoles Female Stem cell Adult stem cell Stem Cell Transplantation |
| Zdroj: | The NOBEL Meeting, Milano, 2010 info:cnr-pdr/source/autori:I. Zucchi, S. Sanzone, S. Astigiano, P. Pelucchi, M. Scotti, V. Valsecchi, O. Barbieri, G. Bertoli, A. Albertini, R. A. Reinbold, and R. Dulbecco./congresso_nome:The NOBEL Meeting/congresso_luogo:Milano/congresso_data:2010/anno:2010/pagina_da:/pagina_a:/intervallo_pagine Proceedings of the National Academy of Sciences of the United States of America 104 (2007): 10476–10481. doi:10.1073/pnas.0703071104 info:cnr-pdr/source/autori:Zucchi I, Sanzone S, Astigiano S, Pelucchi P, Scotti M, Valsecchi V, Barbieri O, Bertoli G, Albertini A, Reinbold RA, Dulbecco R./titolo:The properties of a mammary gland cancer stem cell/doi:10.1073%2Fpnas.0703071104/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2007/pagina_da:10476/pagina_a:10481/intervallo_pagine:10476–10481/volume:104 |
| ISSN: | 0027-8424 |
| Popis: | The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing a single cancer-initiating cell with stem cell properties has proven technically difficult because of the scarcity of the cancer stem cells in the tissue of origin and the lack of specific markers for cancer stem cells. Here we show that a single LA7 cell derived from rat mammary adenocarcinoma has the following properties: the differentiation potential to generate all of the cell lineages of the mammary gland; the ability to generate branched duct-like structures that recapitulate morphologically and functionally the ductal–alveolar-like architecture of the mammary tree; and the capacity to initiate heterogeneous tumors in nonobese diabetic-SCID mice. In addition, we show that cultured cells derived from tumors generated by a single LA7 cell-injection have properties similar to LA7 cells, can generate all of the cell lineages of the mammary gland, and recapitulate the ductal–alveolar-like architecture of the mammary tree. The properties of self-renewal, extensive capacity for proliferation, multilineage differentiation potential, and single-cell tumor-initiation potential suggest that LA7 cells are cancer stem cells and can be used as a model system to study the dynamics of tumor formation at the single-cell level. |
| Databáze: | OpenAIRE |
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