Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
| Autor: | Maria Elisa Calcagnotto, Regina C. Barbosa-Silva, Daisyléa de Souza Paiva, Gabriela Filoso Barnabe, Beatriz M. Longo, Maria Fernanda Valente, Roberto Frussa-Filho, Simone Romariz |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Male
Elevated plus maze medicine.medical_specialty Ganglionic eminence Cell Transplantation Neurogenesis Green Fluorescent Proteins Cell Culture Techniques Biology Anxiety Hippocampus Neuronal precursor cells Rats Sprague-Dawley Cellular and Molecular Neuroscience Neural Stem Cells Cell Movement Interneurons Internal medicine Precursor cell Neurosphere Hipocampo medicine Animals Neuropeptide Y Ansiedade Embryonic Stem Cells Cerebral Cortex Transplantation General Neuroscience Median Eminence Medial ganglionic eminence Ácido gama-aminobutírico Neural stem cell Neurônios Endocrinology Parvalbumins Animals Newborn nervous system Calbindin 2 Transplante biology.protein Exploratory Behavior Rats Transgenic Parvalbumin Research Article |
| Zdroj: | BMC Neuroscience Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
| ISSN: | 1471-2202 |
| DOI: | 10.1186/1471-2202-15-94 |
| Popis: | Background The GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats. Results We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2–3. At early adulthood (PN 62–63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells. Conclusion Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats. Electronic supplementary material The online version of this article (doi:10.1186/1471-2202-15-94) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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