Sequence discrimination by alternatively spliced isoforms of a DNA binding zinc finger domain
| Autor: | Jesse Bolton, Joseph A. Gogos, Tien Hsu, Fotis C. Kafatos |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
HMG-box
Molecular Sequence Data Biology Regulatory Sequences Nucleic Acid Structure-Activity Relationship Animals Drosophila Proteins Protein–DNA interaction Amino Acid Sequence LIM domain Genetics Zinc finger Multidisciplinary Binding Sites Base Sequence Hydrogen Bonding Zinc Fingers Zinc finger nuclease Cell biology RING finger domain DNA binding site DNA-Binding Proteins Alternative Splicing Drosophila melanogaster Oligodeoxyribonucleotides Binding domain Protein Binding Transcription Factors |
| Zdroj: | Science (New York, N.Y.). 257(5078) |
| ISSN: | 0036-8075 |
| Popis: | Two major developmentally regulated isoforms of the Drosophila chorion transcription factor CF2 differ by an extra zinc finger within the DNA binding domain. The preferred DNA binding sites were determined and are distinguished by an internal duplication of TAT in the site recognized by the isoform with the extra finger. The results are consistent with modular interactions between zinc fingers and trinucleotides and also suggest rules for recognition of AT-rich DNA sites by zinc finger proteins. The results show how modular finger interactions with trinucleotides can be used, in conjunction with alternative splicing, to alter the binding specificity and increase the spectrum of sites recognized by a DNA binding domain. Thus, CF2 may potentially regulate distinct sets of target genes during development. |
| Databáze: | OpenAIRE |
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