Influence of Methylenetetrahydrofolate Reductase C677T Gene Polymorphisms in Algerian Infertile Men with Azoospermia or Severe Oligozoospermia
| Autor: | Ouarda Semmame, Dalila Satta, Noureddine Abadi, Douadi Hamane, Mohamed Larbi Rezgoune, Cherifa Benlatreche, Djalila Chellat |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Polymerase Chain Reaction Polymorphism Single Nucleotide Male infertility Genotype medicine Humans Mthfr c677t Allele Nonobstructive azoospermia Gene Alleles Infertility Male Methylenetetrahydrofolate Reductase (NADPH2) Genetics (clinical) DNA Primers Gynecology Azoospermia Base Sequence biology General Medicine medicine.disease Algeria Case-Control Studies Methylenetetrahydrofolate reductase biology.protein Polymorphism Restriction Fragment Length |
| Zdroj: | Genetic Testing and Molecular Biomarkers. 16:874-878 |
| ISSN: | 1945-0257 1945-0265 |
| DOI: | 10.1089/gtmb.2011.0367 |
| Popis: | The C677T allele of the methylenetetrahydrofolate reductase (MTHFR) gene has been suggested to represent a risk factor for male infertility. To confirm this association, the distribution of the single-nucleotide polymorphism C677T was investigated in idiopathic infertile Algerian patients with nonobstructive azoospermia (NOA) or severe oligoasthenoteratozoospermia (OAT). A case-control study was carried out, including 74 idiopathic infertile Algerian patients with NOA (n=46) or severe OAT (n=28) and 84 fertile men as controls. Polymorphism C677T was studied by polymerase chain reaction-restriction fragment length polymorphism, and the results were statistically analyzed.The frequency of genotypes MTHFR 677CC, 677CT, and 677TT in idiopathic infertile men with NOA was 43.48%, 41.30%, and 15.22%; 39.29%, 50%, and 10.71% regarding the severe oligozoospermic men; and 42.86%, 45.24%, and 11.90% in the control group.The data suggest that the C677T MTHFR polymorphism is not a risk factor for idiopathic male subfertility in an Algerian population. |
| Databáze: | OpenAIRE |
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