A water-soluble polysaccharide from the roots of Polygala tenuifolia suppresses ovarian tumor growth and angiogenesis in vivo
Autor: | Hua Yao, Fubin Zhang, Yunduo Liu, Qinghua Tian, Ping Cui, Dan Xu |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A Polygala Angiogenesis Antigens CD34 Apoptosis Pharmacology Biochemistry Plant Roots Ovarian tumor chemistry.chemical_compound Mice 0302 clinical medicine Structural Biology Ovarian Neoplasms Mice Inbred BALB C TUNEL assay Neovascularization Pathologic General Medicine Tumor Burden Vascular endothelial growth factor ErbB Receptors Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis Female medicine.medical_specialty Biology 03 medical and health sciences In vivo Polysaccharides Internal medicine Cell Line Tumor medicine Animals Humans Molecular Biology Cell Proliferation Plant Extracts Water biology.organism_classification medicine.disease Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays 030104 developmental biology Endocrinology chemistry Solubility Polygala tenuifolia Ovarian cancer |
Zdroj: | International journal of biological macromolecules. 107 |
ISSN: | 1879-0003 |
Popis: | PTP, one polysaccharide extracted from the roots of Polygala tenuifolia , has displayed anti-cancer activity in several types of ovarian cancer cells. This study aims to elucidate the structure of PTP and investigate its anticancer effects against SKOV3 xenograft tumor growth in BALB/c mice, as well as the underlying mechanisms involved. GC–MS and NMR data indicate that PTP has a backbone composed of 1,4,6-linked-β-Gal p , 1,4-linked-β-Gal p and 1,4-linked-β-Glc p , with non-reducing terminal 1-linked-α-Glc p attached to O-6 of 1,4,6-linked-β-Gal p . The tumor growth was suppressed in mice following two week’s PTP administration (10, 20 and 40 mg/kg) due to the induction of apoptosis, as detected by TUNEL assay. Moreover, lower serum VEGF and EGFR levels were observed in BALB/c mice treated with different doses of PTP when compared with that in untreated mice. Also, EGFR, VEGF, and CD34 were decreased in both transcript and protein levels in the tumor-bearing mice upon PTP treatment. Taken together, our data suggest that PTP appears to be a powerful chemopreventive agent for the patients with ovarian cancer, especially at advanced stage. |
Databáze: | OpenAIRE |
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