A water-soluble polysaccharide from the roots of Polygala tenuifolia suppresses ovarian tumor growth and angiogenesis in vivo

Autor: Hua Yao, Fubin Zhang, Yunduo Liu, Qinghua Tian, Ping Cui, Dan Xu
Rok vydání: 2017
Předmět:
0301 basic medicine
Vascular Endothelial Growth Factor A
Polygala
Angiogenesis
Antigens
CD34
Apoptosis
Pharmacology
Biochemistry
Plant Roots
Ovarian tumor
chemistry.chemical_compound
Mice
0302 clinical medicine
Structural Biology
Ovarian Neoplasms
Mice
Inbred BALB C
TUNEL assay
Neovascularization
Pathologic
General Medicine
Tumor Burden
Vascular endothelial growth factor
ErbB Receptors
Gene Expression Regulation
Neoplastic
030220 oncology & carcinogenesis
Female
medicine.medical_specialty
Biology
03 medical and health sciences
In vivo
Polysaccharides
Internal medicine
Cell Line
Tumor
medicine
Animals
Humans
Molecular Biology
Cell Proliferation
Plant Extracts
Water
biology.organism_classification
medicine.disease
Antineoplastic Agents
Phytogenic
Xenograft Model Antitumor Assays
030104 developmental biology
Endocrinology
chemistry
Solubility
Polygala tenuifolia
Ovarian cancer
Zdroj: International journal of biological macromolecules. 107
ISSN: 1879-0003
Popis: PTP, one polysaccharide extracted from the roots of Polygala tenuifolia , has displayed anti-cancer activity in several types of ovarian cancer cells. This study aims to elucidate the structure of PTP and investigate its anticancer effects against SKOV3 xenograft tumor growth in BALB/c mice, as well as the underlying mechanisms involved. GC–MS and NMR data indicate that PTP has a backbone composed of 1,4,6-linked-β-Gal p , 1,4-linked-β-Gal p and 1,4-linked-β-Glc p , with non-reducing terminal 1-linked-α-Glc p attached to O-6 of 1,4,6-linked-β-Gal p . The tumor growth was suppressed in mice following two week’s PTP administration (10, 20 and 40 mg/kg) due to the induction of apoptosis, as detected by TUNEL assay. Moreover, lower serum VEGF and EGFR levels were observed in BALB/c mice treated with different doses of PTP when compared with that in untreated mice. Also, EGFR, VEGF, and CD34 were decreased in both transcript and protein levels in the tumor-bearing mice upon PTP treatment. Taken together, our data suggest that PTP appears to be a powerful chemopreventive agent for the patients with ovarian cancer, especially at advanced stage.
Databáze: OpenAIRE
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