Molecular Allergen Profiling of Dual Mite Sensitization in Severe Allergic Rhinitis
| Autor: | M Castillo, Fernando Pineda, Inmaculada Sánchez-Machín, Ruperto González-Pérez, Paloma Poza-Guedes, Victor Matheu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_treatment Immunology Immunoglobulin E medicine.disease_cause Severity of Illness Index Young Adult 03 medical and health sciences 0302 clinical medicine Allergen medicine Mite Animals Humans Immunology and Allergy Clinical significance Antigens Dermatophagoides Sensitization Skin Tests Blomia tropicalis Mites biology business.industry Immunotherapy Allergens Middle Aged biology.organism_classification Serum samples Rhinitis Allergic 030104 developmental biology medicine.anatomical_structure 030228 respiratory system biology.protein Female Immunization business |
| Zdroj: | Journal of Investigational Allergology and Clinical Immunology. 30:421-429 |
| ISSN: | 1018-9068 |
| Popis: | Background: Mites are the most prevalent source of indoor allergens. The present study used a component-resolved diagnosis (CRD) approach to investigate the mite-specific IgE sensitization profile for Dermatophagoides pteronyssinus and Blomia tropicalis. We also assessed the performance of a commercially available CRD approach in patients with severe allergic rhinitis. Methods: We selected 63 consecutive patients with dual sensitization to D pteronyssinus and B tropicalis and persistent severe rhinitis according to the ARIA guidelines. We performed skin prick tests with standardized extracts and determined specific serum IgE to both mites, along with serum specific IgE to Der p 1, Der p 2, Der p 23, Der p 10, and Blo t 5. Results: Fifty-eight and 59 patients had positive sIgE to the whole extracts of D pteronyssinus and B tropicalis, respectively. While 91.67% of patients were sensitized to specific IgE to Der p 1, Der p 2, and/or Der p 23, specific IgE to Blo t 5 (≥0.3 ISU-E) was not detected in most of the serum samples (55%). Conclusions: Although the combination panel of the commercially available major allergens Der p 1, Der p 2, and Der p 23 identified more than 90% of the D pteronyssinus–allergic patients, Blo t 5 performed somewhat poorly in those sensitized to B tropicalis. Improvements in CRD and further research concerning the prevalence and clinical relevance of serodominant allergens are needed to achieve a genuine molecular diagnosis, as well as patient-centered mite allergy–specific immunotherapy. |
| Databáze: | OpenAIRE |
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