Luteinizing Hormone-Releasing Hormone Neurons Express c-fosAntigen after Steroid Activation*
| Autor: | Wen Sen Lee, V. Yann, Gloria E. Hoffman, M. D. Fitzsimmons, Barbara Attardi |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Immunocytochemistry Stimulation Gonadotropin-releasing hormone Biology c-Fos Gonadotropin-Releasing Hormone Endocrinology Proto-Oncogene Proteins Internal medicine medicine Animals Tissue Distribution Progesterone Neurons Estradiol Brain Immunohistochemistry Preoptic area Estrogen biology.protein Luteinizing hormone Proto-Oncogene Proteins c-fos hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Endocrinology. 126:1736-1741 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-126-3-1736 |
| Popis: | Immature female rats received implants containing 17 beta-estradiol on postnatal day 28 at 0900 h, followed 24 h later by either blank capsules or progesterone. Between 1500-1600 h on the day of progesterone (or blank capsule) implantation, these rats, a group of unoperated or sham controls, and a group of estrogen-progesterone-treated immature male rats were killed and perfused, and their brains processed for immunocytochemistry of c-fos antigen and LHRH. LHRH neurons consistently expressed c-fos after estrogen-progesterone treatment in females but not males; in only one of four females examined was c-fos induced after estrogen treatment. No fos was associated with LHRH neurons in the control groups. The LHRH neurons that expressed c-fos were located in the preoptic area and anterior hypothalamus; more rostral LHRH cells did not appear stimulated. These data demonstrate that gonadal steroids, administered in a paradigm that predictably produces timed stimulation of LH release, induce c-fos in LHRH neurons. The induction of c-fos in LHRH neurons provides a potentially useful and powerful tool for studying LHRH activation at the cellular level. |
| Databáze: | OpenAIRE |
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