High performance liquid chromatographic determination of 1,2-[bis(1,2-benzisoselenazolone-3(2H)-ketone)]-ethane (BBSKE), a novel organoselenium compound, in dog plasma using pre-column derivatization and its application in pharmacokinetic study
| Autor: | Guo-Liang Zhang, Gui-Fang Dou, Ya-Qing Lou, Zhi-Yun Meng, Hai-Yan Zhou |
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| Rok vydání: | 2007 |
| Předmět: |
Male
chemistry.chemical_classification Chromatography Ketone Clinical Biochemistry Reproducibility of Results Cell Biology General Medicine Bridged Bicyclo Compounds Heterocyclic Mass spectrometry Sensitivity and Specificity Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Dogs chemistry Linear range Pharmacokinetics Organoselenium Compounds Animals Derivatization 2-Mercaptoethanol Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B. 852:617-624 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2007.02.040 |
| Popis: | A novel HPLC-UV method with pre-column derivatization by using 2-mercaptoethanol was established for determination of 1,2-[bis(1,2-benzisoselenazolone-3(2H)-ketone)]-ethane (BBSKE) in dog plasma. The derivatives were identified by mass spectrometry. The method had a good linear range of 0.05-2 microg/ml (r(2)=0.9995). The lower limit of quantification (LOQ) was 0.05 microg/ml. The precision and accuracy were less than 7%. After dosing of BBSKE (30 mg/kg, p.o. and 0.79 mg/kg, i.v.) in dogs, AUC(0-t) were 5.72+/-2.42 and 1.35+/-0.41 microg h/ml; t(1/2) were 4.6+/-2.1 and 1.7+/-0.6h, respectively. The method was successfully applied to the pharmacokinetic study in dogs. |
| Databáze: | OpenAIRE |
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