Targeting proapoptotic protein BAD inhibits survival and self-renewal of cancer stem cells
Autor: | M K Al-Kowari, Dhanya Kizhakayil, Lotfi Chouchane, A Haoudi, A Ismail Chouchane, Pu Li, George Kulik, Ena Wang, M A Al-Muftah, Konduru S Sastry, Francesco M. Marincola |
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Rok vydání: | 2014 |
Předmět: |
Male
Cell Survival Population Apoptosis Breast Neoplasms Biology Prostate cancer Cancer stem cell Cell Line Tumor medicine Humans education Molecular Biology Cell Proliferation Original Paper education.field_of_study Melanoma Cell Cycle CD44 Prostatic Neoplasms Cell Biology Cell cycle medicine.disease Tumor progression Gene Knockdown Techniques Cancer cell Immunology Neoplastic Stem Cells Cancer research biology.protein Female bcl-Associated Death Protein Protein Processing Post-Translational |
Zdroj: | Cell Death & Differentiation. 21:1936-1949 |
ISSN: | 1476-5403 1350-9047 |
Popis: | Emerging evidence suggests that the resistance of cancer stem cells (CSC) to many conventional therapies is one of the major limiting factors of cancer therapy efficacy. Identification of mechanisms responsible for survival and self-renewal of CSC will help design new therapeutic strategies that target and eliminate both differentiated cancer cells and CSC. Here we demonstrated the potential role of proapoptotic protein BAD in the biology of CSC in melanoma, prostate and breast cancers. We enriched CD44(+)/CD24(-) cells (CSC) by tumorosphere formation and purified this population by FACS. Both spheres and CSC exhibited increased potential for proliferation, migration, invasion, sphere formation, anchorage-independent growth, as well as upregulation of several stem cell-associated markers. We showed that the phosphorylation of BAD is essential for the survival of CSC. Conversely, ectopic expression of a phosphorylation-deficient mutant BAD induced apoptosis in CSC. This effect was enhanced by treatment with a BH3-mimetic, ABT-737. Both pharmacological agents that inhibit survival kinases and growth factors that are involved in drug resistance delivered their respective cytotoxic and protective effects by modulating the BAD phosphorylation in CSC. Furthermore, the frequency and self-renewal capacity of CSC was significantly reduced by knocking down the BAD expression. Consistent with our in vitro results, significant phosphorylation of BAD was found in CD44(+) CSC of 83% breast tumor specimens. In addition, we also identified a positive correlation between BAD expression and disease stage in prostate cancer, suggesting a role of BAD in tumor advancement. Our studies unveil the role of BAD in the survival and self-renewal of CSC and propose BAD not only as an attractive target for cancer therapy but also as a marker of tumor progression. |
Databáze: | OpenAIRE |
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